Odor-driven activity in the olfactory cortex of an in vitro isolated guinea pig whole brain with olfactory epithelium
Odor-driven activity in the olfactory cortex of an in vitro isolated guinea pig whole brain with olfactory epithelium
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DOI：
10.1152/jn.01366.2005
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发表时间：
2007-01-01
影响因子：
2.5
通讯作者：
Iijima, Toshio
中科院分区：
文献类型：
--
作者：
Ishikawa, Takahiro;Sato, Takaaki;Iijima, Toshio
石川、Takahiro、Takaaki Sato、Akira Shimizu、Ken-Ichiro Tsutsui、Marco de Curtis和Toshio Iijima。离体豚鼠全脑嗅觉皮层的气味驱动活动。J Neurophysiol 97：670-679，2007.首次发表于2006年7月26日; doi：10.1152/jn.01366.2005。我们开发了一种新技术来分离具有完整嗅觉上皮（OE）的整个豚鼠大脑，使我们能够在自然气味刺激期间轻松进入大脑的腹侧表面，包括嗅觉区域。我们将气味剂应用于OE，并证实气味诱导的局部场电位（LFPs）可以在嗅觉区诱导。在嗅球（OB）和梨状皮质（PC），气味诱导的LFPs包括一个阶段性的初始组件，然后在β范围内（20 Hz）的快速活动振荡。为了了解气味诱导反应的神经机制，特别是在前PC，我们分析了气味诱导的LFPs，连同单位活动数据。我们证实，气味诱导的反应的初始组件具有一个特征的时间模式，由一个相对较弱的直接传入输入，然后由皮层内的联想反应，这是与相位抑制。贝塔振荡可能是由这些网络活动的重复形成的。这些电生理数据与以前使用切片或体内制剂的研究结果一致，表明嗅觉神经网络和大脑活动在我们新的体外制剂中得以保留。本研究为阐明自然嗅觉刺激后离体脑中气味信息加工的神经活动序列提供了基础。
Ishikawa, Takahiro, Takaaki Sato, Akira Shimizu, Ken-Ichiro Tsutsui, Marco de Curtis, and Toshio Iijima. Odor-driven activity in the olfactory cortex of an in vitro isolated guinea pig whole brain with olfactory epithelium. J Neurophysiol 97: 670-679, 2007. First published July 26, 2006; doi:10.1152/jn.01366.2005. We developed a new technique to isolate a whole guinea pig brain with an intact olfactory epithelium (OE) that enables us to access the ventral surface of the brain including olfactory areas with ease during natural odor stimulation. We applied odorants to OE and confirmed that odorinduced local field potentials (LFPs) could be induced in olfactory areas. In the olfactory bulb (OB) and the piriform cortex (PC), odor-induced LFPs consisted of a phasic initial component followed by a fast activity oscillation in the beta range (20 Hz). To understand the neural mechanisms of odor-induced responses especially in the anterior PC, we analyzed odor-induced LFPs, together with unit activity data. We confirmed that the initial component of odor-induced response has a characteristic temporal pattern, generated by a relatively weak direct afferent input, followed by an intra-cortical associative response, which was associated with a phasic inhibition. The beta oscillation might be formed by the repetition of these network activities. These electrophysiological data were consistent with the results of previous studies that used slice or in vivo preparations, suggesting that the olfactory neural network and activities of the brain are preserved in our new in vitro preparation. This study provides the basis for clarifying the sequence of neural activities underlying odor information processing in the brain in vitro following natural olfactory stimulation.