Anti-adhesion of probiotic Enterococcus faecium WEFA23 against five pathogens and the beneficial effect of its S-layer proteins against Listeria monocytogenes
Anti-adhesion of probiotic Enterococcus faecium WEFA23 against five pathogens and the beneficial effect of its S-layer proteins against Listeria monocytogenes
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益生菌屎肠球菌 WEFA23 对五种病原体的抗粘附作用及其 S 层蛋白对单核细胞增生李斯特氏菌的有益作用
DOI：
10.1139/cjm-2018-0031
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发表时间：
2019-03-01
影响因子：
2.8
通讯作者：
Wei, Hua
中科院分区：
文献类型：
--
作者：
He, Yao;Xu, Xiongpeng;Wei, Hua
粪肠球菌WEFA23是一种从中国婴儿粪便中分离出来的潜在益生菌。在这项研究中，研究了粪肠球菌WEFA23对病原体粘附的拮抗活性。肠球菌WEFA23能够竞争，排除和取代大肠杆菌O157：H7，Typhimurium atcc 13311，单核细胞增生李斯特菌单核细胞增生李斯特氏菌CMCC54007，CMCC26003和Shigella-Sonnei-sonnei-sonnei-sonnei-sonnei-sonnei-sonnei-sonnei-sonnei-sonnei。其中，单核细胞增生乳杆菌在竞争和位移测定中达到了最强的抑制率。这些抗粘附能力与细菌性化学特性（疏水性，自身聚集和聚集）有关。对于单核细胞增生乳杆菌，抗粘附能力受热处理，细胞密度和生长期的影响；在固定期，每毫升的108个菌落形成单位表现出最强的抗粘附活性。此外，用5 mol/L LICL处理去除粪肠球菌WEFA23的S层蛋白质显着降低了其粘附能力，这些S层蛋白能够在不同水平的不同水平上竞争，取代和排除单核基因。粪肠球菌WEFA23的细胞和S层蛋白都显着降低了由单核细胞增生李斯特氏菌诱导的Caco-2细胞的凋亡，这是由Caspase-3激活介导的。这项研究可能有助于理解益生菌针对病原体的抗粘附机制。
Enterococcus faecium WEFA23 is a potential probiotic strain isolated from Chinese infant feces. In this study, the antagonistic activity of E. faecium WEFA23 on adhesion to pathogens was investigated. Enterococcus faecium WEFA23 was able to compete, exclude, and displace the adhesion of Escherichia coli O157:H7, Salmonella Typhimurium ATCC 13311, Listeria monocytogenes CMCC54007, Staphylococcus aureus CMCC26003, and Shigella sonnei ATCC 25931 to Caco-2 cells. Among them, L. monocytogenes achieved the strongest inhibition rate in both competition and displacement assays. Those anti-adhesion capacities were related to the bacterial physicochemical properties (hydrophobicity, auto-aggregation, and co-aggregation) of the bacterial surface. For L. monocytogenes, the anti-adhesion capacity was affected by the heat treatment, cell density, and growth phase of E. faecium WEFA23; 108 colony-forming units of viable cells per millilitre at the stationary phase exhibited the strongest anti-adhesion activity. In addition, removal of S-layer proteins of E. faecium WEFA23 by treatment with 5 mol/L LiCl significantly decreased its adhesion capacity, and those S-layer proteins were able to compete, displace, and exclude L. monocytogenes at different levels. Both cells and S-layer proteins of E. faecium WEFA23 significantly reduced the apoptosis of Caco-2 cells induced by L. monocytogenes, which was mediated by caspase-3 activation. This study might be helpful in understanding the anti-adhesion mechanism of probiotics against pathogens.