Heteromeric Anopheline Odorant Receptors Exhibit Distinct Channel Properties
Heteromeric Anopheline Odorant Receptors Exhibit Distinct Channel Properties
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DOI：
10.1371/journal.pone.0028774
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发表时间：
2011-12-09
期刊：
影响因子：
3.7
通讯作者：
Zwiebel, Laurence J.
中科院分区：
文献类型：
--
作者：
Pask, Gregory M.;Jones, Patrick L.;Zwiebel, Laurence J.
Background: Insect odorant receptors (ORs) function as odorant-gated ion channels consisting of a conventional, odorant-binding OR and the Orco coreceptor. While Orco can function as a homomeric ion channel, the role(s) of the conventional OR in heteromeric OR complexes has largely focused only on odorant recognition.Results: To investigate other roles of odorant-binding ORs, we have employed patch clamp electrophysiology to investigate the properties of the channel pore of several OR complexes formed by a range of different odorant-specific Anopheles gambiae ORs (AgOrs) each paired with AgOrco. These studies reveal significant differences in cation permeability and ruthenium red susceptibility among different AgOr complexes.Conclusions: With observable differences in channel function, the data support a model in which the odorant-binding OR also affects the channel pore. The variable effect contributed by the conventional OR on the conductive properties of odorant-gated sensory channels adds additional complexity to insect olfactory signaling, with differences in odor coding beginning with ORs on the periphery of the olfactory system.
