RNAi knockdown of acetyl-CoA carboxylase gene eliminates jinggangmycin-enhanced reproduction and population growth in the brown planthopper, Nilaparvata lugens.
RNAi knockdown of acetyl-CoA carboxylase gene eliminates jinggangmycin-enhanced reproduction and population growth in the brown planthopper, Nilaparvata lugens.
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乙酰辅酶A羧化酶基因的RNAi敲低消除了井冈霉素增强的褐飞虱Nilaparvata lugens的繁殖和种群生长。
DOI：
10.1038/srep15360
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发表时间：
2015-10-20
影响因子：
4.6
通讯作者：
Wu JC
中科院分区：
文献类型：
--
作者：
Zhang YX;Ge LQ;Jiang YP;Lu XL;Li X;Stanley D;Song QS;Wu JC
生态学中的一个主要挑战在于理解在生物体水平上有很好记录的intraguild物种的共存，但不是在分子水平上。本文研究了井冈霉素（JGM）对褐飞虱（Nilaparvata lugens）和白背飞虱（Sogatella furcifera）繁殖的影响。JGM暴露显着增加BPH的繁殖力和人口增长，但抑制实验室和现场白背飞虱种群的参数。我们使用数字基因表达和转录组学分析来鉴定一组差异表达的基因，包括一组在JGM处理的BPH中上调的基因，这些基因在JGM处理的WBPH中下调。乙酰辅酶A羧化酶（ACC）在JGM处理的BPH中高度表达，其RNAi沉默降低了ACC表达（> 60%），并消除了JGM诱导的BPH生殖力增加。这些发现支持了我们的假设，即ACC表达的差异在分子水平上分离了intraguild物种。
A major challenge in ecology lies in understanding the coexistence of intraguild species, well documented at the organismal level, but not at the molecular level. This study focused on the effects of the antibiotic, jinggangmycin (JGM), a fungicide widely used in Asian rice agroecosystems, on reproduction of insects within the planthopper guild, including the brown planthopper (BPH) Nilaparvata lugens and the white-backed planthopper (WBPH) Sogatella furcifera, both serious resurgence rice pests. JGM exposure significantly increased BPH fecundity and population growth, but suppressed both parameters in laboratory and field WBPH populations. We used digital gene expression and transcriptomic analyses to identify a panel of differentially expressed genes, including a set of up-regulated genes in JGM-treated BPH, which were down-regulated in JGM-treated WBPH. RNAi silencing of Acetyl Co-A carboxylase (ACC), highly expressed in JGM-treated BPH, reduced ACC expression (by > 60%) and eliminated JGM-induced fecundity increases in BPH. These findings support our hypothesis that differences in ACC expression separates intraguild species at the molecular level.