RNase E-dependent processing stabilizes MicX, a Vibrio cholerae sRNA
RNase E-dependent processing stabilizes MicX, a Vibrio cholerae sRNA
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DOI：
10.1111/j.1365-2958.2007.05796.x
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发表时间：
2007-07-01
影响因子：
3.6
通讯作者：
Waldor, Matthew K.
中科院分区：
文献类型：
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作者：
Davis, Brigid M.;Waldor, Matthew K.
在霍乱弧菌中，生物信息学方法已被用于预测许多小RNA（sRNA）编码基因的位置，但生物学作用已被确定的很少。在这里，我们描述了通过这种分析鉴定的sRNA（以前称为A10）的表达，加工和生物学作用。我们将这种sRNA重新命名为MicX，就像大肠杆菌sRNA云母，MicC和MicF一样，它调节外膜蛋白（OMP）的表达。MicX似乎是vc 0972和vc 0620的直接负调节因子，vc 0972编码未表征的OMP，vc 0620编码肽ABC转运蛋白的周质组分。Hfq显然不是MicX与这些靶标相互作用和调节所必需的。MicX的编码序列与vca 0943重叠;然而，MicX的初级转录物以RNase E-和Hfq-依赖的方式被加工成更短、仍然有活性和更稳定的形式，主要由vca 0943 3 3'非翻译区组成。我们的数据表明，MicX的加工增强了其有效性，并且sRNA切割不仅仅是sRNA失活和清除的手段。
In Vibrio cholerae, bioinformatic approaches have been used to predict the locations of numerous small RNA (sRNA)-encoding genes, but biological roles have been determined for very few. Here, we describe the expression, processing and biological role of an sRNA (previously known as A10) that was identified through such analyses. We have renamed this sRNA MicX as, like the Escherichia coli sRNAs MicA, MicC and MicF, it regulates expression of an outer membrane protein (OMP). MicX appears to be a direct negative regulator of vc0972, which encodes an uncharacterized OMP, and vc0620, which encodes the periplasmic component of a peptide ABC transporter. Hfq is apparently not required for MicX's interactions with and regulation of these targets. The sequence encoding MicX overlaps with vca0943; however, primary transcripts of MicX are processed in an RNase E- and Hfq-dependent fashion to a shorter, still active and much more stable form consisting largely of the vca0943 3' untranslated region. Our data suggest that processing of MicX enhances its effectiveness, and that sRNA cleavage is not simply a means to sRNA inactivation and clearance.