The Toxic Effect of Ketamine on SH-SY5Y Neuroblastoma Cell Line and Human Neuron
The Toxic Effect of Ketamine on SH-SY5Y Neuroblastoma Cell Line and Human Neuron
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DOI：
10.1002/jemt.20774
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发表时间：
2010-03-01
影响因子：
2.5
通讯作者：
Yew, David T.
中科院分区：
文献类型：
--
作者：
Mak, Ying T.;Lam, Wai P.;Yew, David T.
氯胺酮在人类和动物医学中用作注射麻醉剂，也是一种娱乐药物，主要由年轻人使用，经常在夜总会的夜间舞会上使用。在过去的5年里，全世界氯胺酮使用者的比例增长非常快。然而，这导致了一个严重的问题，即氯胺酮对我们的神经系统，特别是大脑的长期不利影响，因为作为NMDA拮抗剂，氯胺酮可能会导致神经元凋亡。因此，本研究旨在探讨氯胺酮对神经细胞长期孵育(48h)的慢性影响。结果表明，分化的神经细胞容易受到氯胺酮的毒性，但可能比未分化的神经细胞和成纤维细胞更不敏感。这表明，滥用氯胺酮对许多其他器官和大脑都是有害的。我们的结果也证实了氯胺酮的毒性作用与分化后的神经细胞通过Bax/Bcl2比值通路和caspase-3的凋亡有关。因此，应寻求长期氯胺酮处理的细胞或动物模型来研究氯胺酮的这种多器官效应。Microsc.技术资源。73：195-201,2010。(C)Wiley-Liss Inc.
Ketamine used as an injectable anesthetic in human and animal medicine is also a recreational drug used primarily by young adults often at all night dance parties in nightclubs. The percentage of ketamine users has grown very fast in the last 5 years worldwide. However, this leads to the serious question of the long-term adverse effects of ketamine on our nervous system, particularly the brain, because ketamine as an NMDA antagonist could Cause neurons to commit apoptosis. Our study therefore aimed to find out the chronic effect of ketamine on neuron using prolonged incubation (48 h) of neuronal cells with ketamine in culture. Out, results showed that differentiated neuronal cells were prone to the toxicity of ketamine but probably less susceptible than undifferentiated neuronal cells and fibroblasts. This suggested that the ketamine abuse would be harmful to many other organs as well as the brain. Our results also confirmed that the toxicity of ketamine is related to apoptosis via the Bax/Bcl-2 ratio pathway and caspase-3 in the differentiated neuronal cells. Therefore, long-term ketamine treated cell or animal models should be sought to study this multiorgan effects of ketamine. Microsc. Res. Tech. 73:195-201, 2010. (c) Wiley-Liss Inc.