Two new balancer chromosomes on mouse chromosome 4 to facilitate functional annotation of human chromosome 1p
Two new balancer chromosomes on mouse chromosome 4 to facilitate functional annotation of human chromosome 1p
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DOI：
10.1002/gene.10207
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发表时间：
2003-07-01
期刊：
影响因子：
1.5
通讯作者：
Bradley, A
中科院分区：
文献类型：
--
作者：
Nishijima, I;Mills, A;Bradley, A
为了便于遗传筛选以鉴定和维持映射到人类1号染色体短臂的隐性突变，我们利用染色体工程产生了两种在小鼠4号染色体远端区域携带大倒位的小鼠品系。倒位间隔的大小为16和22 cM，它们一起覆盖了大约一半的4号染色体。由于野生型和倒位染色体之间的重组不会发生在这些倒位间隔内，因此可以鉴定和维持映射到该区域的基因的突变等位基因。因此，这些倒位染色体起平衡染色体的作用。这些倒位具有额外的优点，即它们用编码可见毛色标记物酪氨酸酶和琼脂糖蛋白的基因标记，因此可以视觉识别倒位染色体（+/+，Inv/+，Inv/Inv）的剂量。这些倒位菌株将是非常有用的诱变筛选，专注于人类染色体1 p的功能注释。创世纪36：142-148，2003。(C)2003 Wiley-Liss，Inc.
To facilitate genetic screens to identify and maintain recessive mutations that map to the short arm of human chromosome 1, we have utilized chromosome engineering to generate two mouse strains that carry large inversions on the distal region of mouse chromosome 4. The inversion intervals are 16 and 22 cM in size together they cover approximately half of chromosome 4. Since recombination between the wild-type and inversion chromosomes does not occur within these inversion intervals, mutant alleles of genes mapping to this region can be identified and maintained. Therefore, these inversion chromosomes work as balancer chromosomes. These inversions have the additional advantage that they are tagged with genes encoding the visible coat color markers tyrosinase and agouti, and therefore the dosage of the inversion chromosome (+/+, Inv/+, Inv/Inv) can be visually recognized. These inversion strains will be extremely useful for mutagenesis screens that focus on functional annotation of human chromosome 1p. genesis 36:142-148, 2003. (C) 2003 Wiley-Liss, Inc.