Growth inhibition of glioma cells transfected with the human beta-interferon gene by liposomes coupled with a monoclonal antibody.
Growth inhibition of glioma cells transfected with the human beta-interferon gene by liposomes coupled with a monoclonal antibody.
复制标题
通过与单克隆抗体偶联的脂质体对转染人β-干扰素基因的神经胶质瘤细胞的生长抑制。
作者：
M. Mizuno;J. Yoshida;K. Sugita;I. Inoue;H. Seo;Y. Hayashi;T. Koshizaka;K. Yagi
A human beta-interferon (HuIFN-beta) gene inserted into a eukaryotic expression vector (pSV2IFN-beta) was entrapped in liposomes having positive charges on their surface. Liposome-mediated transfection of the gene into cultured glioma cells (U251-MG) resulted in the secretion of HuIFN-beta into the medium. The HuIFN-beta level in the culture medium of glioma cells reached 24 +/- 8 (mean +/- SD) IU/ml after 96 h of incubation, at which level the growth inhibitory effect on the cells was found to be greater than 40 times as compared with exogenously added HuIFN-beta. When the plasmid-containing liposomes were coupled with a monoclonal antibody (G-22 MCA) against glioma-associated antigen, the level of HuIFN-beta in the medium was 178 +/- 26 IU/ml, resulting in a 7-fold increase, and the growth inhibitory effect was further elevated. Since the addition of a monoclonal antibody against HuIFN-beta to the medium did not cause the cell growth to resume, the growth inhibitory effect on the cells seems to be ascribed to HuIFN-beta produced in the cells transfected with its gene. Accordingly, the specific delivery of the HuIFN-beta gene into glioma cells by the use of such liposomes might become a useful technique for gene therapy of malignant glioma.
DOI：
10.1016/s0021-9258(19)70482-7
发表时间：
1980-11
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Fraley;S. Subramani;P. Berg;D. Papahadjopoulos
通讯作者：
R. Fraley;S. Subramani;P. Berg;D. Papahadjopoulos
选择性递送封装在脂质体中的药物：自然靶向参与各种疾病状态的巨噬细胞。
DOI：
--
发表时间：
1983
期刊：
Journal of biological response modifiers
影响因子：
--
作者：
Schroit,AJ;Hart,IR;Madsen,J;Fidler,IJ
通讯作者：
Fidler,IJ
影响因子：
2.9
作者：
FRALEY, R;STRAUBINGER, RM;PAPAHADJOPOULOS, D
通讯作者：
PAPAHADJOPOULOS, D