Iron single-atom anchored N-doped carbon as a 'laccase-like' nanozyme for the degradation and detection of phenolic pollutants and adrenaline
Iron single-atom anchored N-doped carbon as a 'laccase-like' nanozyme for the degradation and detection of phenolic pollutants and adrenaline
复制标题
DOI：
10.1016/j.jhazmat.2021.127763
复制
发表时间：
2021-12-30
影响因子：
13.6
通讯作者：
Lu, Guo-Ping
中科院分区：
文献类型：
--
作者：
Lin, Yamei;Wang, Fei;Lu, Guo-Ping
为了解决漆酶的固有缺陷，公开了作为漆酶模拟物的第一种铁单原子锚定的N掺杂碳材料（Fe 1@CN 20）。该材料的FeN 4结构可以很好地模拟漆酶的催化活性。尽管Fe1@CN-20的金属含量（2.9wt%）低于任何先前报道的漆酶模拟物，但它表现出与漆酶相当的动力学常数，因为它的Km（米氏常数）和Vmax（最大速率）分别为0.070 mM和2.25 μ M/min，这与漆酶（0.078 mM，2.49 μ M/ min）相似。该催化剂即使在极端pH（2-9）、高温（100 ℃）、强离子强度（500 mM NaCl）、高乙醇浓度（体积比40%）和长储存时间（2个月）下也显示出优异的稳定性。此外，它可以重复使用至少7次，只有轻微的活性损失。因此，这种材料具有比漆酶低得多的价格和更好的稳定性和可回收性，已被应用于一系列酚类化合物的检测和降解。在肾上腺素的检测中，Fe1@CN-20达到了1.3 μ M的检测限，表明它比漆酶（3.9 μ M）更敏感。
To solve the inherent defects of laccase, the first iron single-atom anchored N-doped carbon material (Fe1@CN20) as a laccase mimic was disclosed. The FeN4 structure of this material can well mimic the catalytic activity of laccase. Although Fe1@CN-20 has a lower metal content (2.9 wt%) than any previously reported laccase mimics, it exhibits kinetic constants comparable to those of laccase, as its Km (Michaelis constant) and Vmax (maximum rate) are 0.070 mM and 2.25 mu M/min respectively, which are similar to those of laccase (0.078 mM, 2.49 mu M/ min). This catalyst displays excellent stability even under extreme pH (2-9), high temperature (100 degrees C), strong ionic strength (500 mM of NaCl), high ethanol concentration (volume ratio 40%) and long storage time (2 months). Additionally, it can be reused for at least 7 times with only a slight loss in activity. Therefore, this material has a much lower price and better stability and recyclability than laccase, which has been applied in the detection and degradation of a series of phenolic compounds. In the detection of adrenaline, Fe1@CN-20 achieved a detection limit of 1.3 mu M, indicating it is more sensitive than laccase (3.9 mu M).