Agarose-embedded tissue arrays for histologic and genetic analysis
Agarose-embedded tissue arrays for histologic and genetic analysis
复制标题
DOI：
10.2144/98254st02
复制
发表时间：
1998-10-01
期刊：
影响因子：
2.7
通讯作者：
Cheng, KC
中科院分区：
文献类型：
--
作者：
Tsao-Wu, GS;Weber, CH;Cheng, KC
为了便于对大量7天大的斑马鱼（Danio rerio）进行组织学分析，开发了一种在琼脂糖包埋阵列中处理它们的方法。使用薄的组织切片，可以在显微镜下检查细胞和组织的形态，以研究各种生物过程。由于其体积小，需要精确排列幼虫以同时切片。设计了一种技术，在切片之前将斑马鱼幼虫组包埋在琼脂糖中的单个平面中。使用这种包埋方法可以有效地检查数千只幼虫的染色组织切片。除了组织学分析，也可以对个体幼虫切片的DNA进行基于PCR的基因型分析。该技术可以修改，以适应任何研究，需要组织学检查的许多块组织。
To facilitate the histologic analysis of large numbers of 7-day-old zebrafish (Danio rerio), a method has been developed to process them in agarose-embedded arrays. Using thin tissue sections, the morphology of cells and tissues can be examined microscopically to investigate a variety of biologic processes. Because of their small size, precise arrangement of the larvae is necessary to section them simultaneously. A technique was designed to embed groups of zebrafish larvae in a single plane in agarose before sectioning. Stained tissue sections of thousands of larvae can be examined efficiently using this embedding method. In addition to histologic analysis, PCR-based genotypic analysis of DNA from individual larval sections is also possible. This technique can be modified to accommodate any study that requires the histologic examination of many pieces of tissue.