Impairment of the mitochondrial electron transport chain due to sleep deprivation in mice
Impairment of the mitochondrial electron transport chain due to sleep deprivation in mice
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DOI：
10.1016/j.jpsychires.2010.01.015
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发表时间：
2010-09-01
影响因子：
4.8
通讯作者：
Tufik, Sergio
中科院分区：
文献类型：
--
作者：
Andreazza, Ana C.;Andersen, Monica L.;Tufik, Sergio
已经证明，睡眠剥夺与代谢过程、对压力和炎症的反应、昼夜睡眠/觉醒周期、细胞增殖调节和各种信号传导途径相关的基因表达改变有关。然而，这些变化背后的分子机制仍然知之甚少。因此，本研究的目的是使用一个动物模型的反常睡眠剥夺（PSD）的特点在大脑中的线粒体电子传递链的功能。睡眠恢复（反弹）是否可以逆转PSD后发现的变化的问题也得到了解决。将成年雄性近交系C57BL/6 J小鼠随机分为3组：笼内对照组、PSD组和睡眠反弹组。PSD组和反跳组均行PSD处理72 h。在该睡眠剥夺期后，将反弹组放回其饲养笼中，并允许其以不受干扰和自发的方式睡眠24小时。线粒体复合物复合物II，琥珀酸脱氢酶和复合物II-III的活动，然后通过分光光度法测定提取的前额叶皮层，海马，纹状体和下丘脑的亚线粒体颗粒。我们的结果表明，在PSD和反弹组的复合物的活性显着降低，与对照组相比。复合物II和II-III活性在睡眠反弹组的下丘脑中特别降低。这些结果与睡眠参与能量代谢一致，并证实了先前的实验表明下丘脑在睡眠调节中的重要性。(C)2010爱思唯尔有限公司版权所有。
It has been demonstrated that sleep deprivation is associated with altered expression of genes related to metabolic processes, response to stress and inflammation, circadian sleep/wake cycles, regulation of cell proliferation and various signaling pathways. However, the molecular mechanisms underlying these changes remain poorly understood. Thus, the present study aims to characterize the function of the mitochondrial electron transport chain in the brain using an animal model of paradoxical sleep deprivation (PSD). The question of whether sleep recovery (rebound) can reverse changes found after PSD is also addressed. Adult male inbred C57BL/6 J mice were randomly distributed into three groups: home-cage control, PSD and sleep rebound groups. The PSD and rebound groups were subjected to PSD for 72 h. After this sleep deprivation period, the rebound group was returned to its home cage and allowed to sleep in an undisturbed and spontaneous fashion for 24 h. The mitochondrial complex complex II, succinate dehydrogenase and complex II-III activities were then measured by spectrophotometric methods in sub-mitochondrial particles extracted from the prefrontal cortex, hippocampus, striatum and hypothalamus. Our results showed a significant decrease in the activity of complex in the PSD and rebound groups as compared to the control group. The complex II and II-III activity were particularly decreased in the hypothalamus of the sleep rebound group. These results are consistent with the involvement of sleep in energy metabolism and corroborate previous experiments demonstrating the importance of the hypothalamus in sleep regulation. (C) 2010 Elsevier Ltd. All rights reserved.