Nanospray "taxation' and how to avoid it
Nanospray "taxation' and how to avoid it
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DOI：
10.1002/rcm.1649
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发表时间：
2004-01-01
影响因子：
2
通讯作者：
Karas, M
中科院分区：
文献类型：
--
作者：
Chernushevich, IV;Bahr, U;Karas, M
In mass spectrometric analysis with nanospray ionization, some analytes were found to appear in spectra with a delay of tens of minutes, while a few others could not be detected at all. The effect was found to be related to cation-exchange chromatography with negative charge on the glass surface, and with the most affected peptide or protein ions having strong localization of positive charge in blocks of two or more adjacent basic amino acid residues (e.g. melittin). The 'affinity' to the glass surface was studied with a peptide mixture and bovine serum albumin (BSA) tryptic digest solutions at sub-micromolar concentration. About 20% fewer tryptic peptides could be identified from spectra recorded with a glass nanospray capillary compared to those acquired with either conventional 1 muL/min electrospray or a quartz nanospray capillary. Protein identification studies are not likely to be seriously affected by this loss, but other protein applications, such as investigations of mutations or post-translational modifications, may suffer due to reduced sequence coverage. Ways to avoid losses of useful ions are discussed. Copyright (C) 2004 John Wiley Sons, Ltd.
