Evaluation of Remel Spectra CRE Agar for Detection of Carbapenem-Resistant Bacteria from Rectal Swabs Obtained from Residents of a Long-Term-Care Facility.

Evaluation of Remel Spectra CRE Agar for Detection of Carbapenem-Resistant Bacteria from Rectal Swabs Obtained from Residents of a Long-Term-Care Facility.
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对 Remel Spectra CRE 琼脂用于检测长期护理机构居民直肠拭子中的碳青霉烯类耐药细菌的评估。

DOI:
10.1128/jcm.00789-15
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发表时间:
2015
影响因子:
9.4
通讯作者:
Segal-Maurer,Sorana
Segal-Maurer,Sorana
中科院分区:
医学2区
文献类型:
--
作者:
LaBombardi,VincentJ;Urban,CarlM;Kreiswirth,BarryN;Chen,Liang;Osorio,Giuliana;Kopacz,Joanna;Labaze,Georges;Segal-Maurer,Sorana

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我们比较了Remel Spectra CRE琼脂平板与CDC标准方法,用于从长期护理机构(LTCF)患者的300份直肠拭子标本中分离碳青霉烯类耐药肠杆菌科(CRE)。对分离株进行多重PCR实验,以鉴定特异性肺炎克雷伯菌碳青霉烯酶(KPC)和其他β-内酰胺酶。在300例患者中,72例(24%)患有CRE,且KPC酶PCR阳性。Remel Spectra CRE平板在72例患者中的70例(97.2%)分离株中检出KPC型CRE,而CDC方法在72例患者中的56例(77.8%)分离株中检出CRE。CRE鉴定结果可在18小时内获得,而CDC方法为36小时。Remel Spectra CRE琼脂平板可为快速可靠检测KPC型CRE的方法以及加速制定适当的感染控制预防措施提供有用的手段。
We compared the Remel Spectra CRE agar plate to CDC standard methodology for the isolation of carbapenem-resistant Enterobacteriaceae (CRE) from 300 rectal swab specimens obtained from patients residing in a long-term-care facility (LTCF). Multiplex PCR experiments were performed on isolates to identify specific Klebsiella pneumoniae carbapenemases (KPC) and additional β-lactamases. Of the 300 patients, 72 (24%) harbored CRE and were PCR positive for KPC enzymes. The Remel Spectra CRE plates detected KPC-type CRE in isolates from 70 of 72 patients (97.2%), while the CDC method detected CRE in 56 of 72 (77.8%). CRE identification results were available in 18 h compared to 36 h for the CDC method. Remel Spectra CRE agar plates can provide useful means for a fast and reliable method for detecting KPC-type CRE and for accelerated institution of appropriate infection control precautions.
脑去甲肾上腺素在抗精神病药引起的大鼠僵直症中的作用
DOI: --
发表时间: 1977
期刊: Pharmacology, Biochemistry and Behavior
影响因子: --
作者:
T. Honma;H. Fukushima
通讯作者: H. Fukushima