Borrelia burgdorferi are susceptible to killing by a variety of human polymorphonuclear leukocyte components.
Borrelia burgdorferi are susceptible to killing by a variety of human polymorphonuclear leukocyte components.
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伯氏疏螺旋体易被多种人类多形核白细胞成分杀死。
DOI：
10.1086/339341
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Montgomery,RuthR
中科院分区：
文献类型：
--
作者：
Lusitani,Denise;Malawista,StephenE;Montgomery,RuthR
比较了完整的人多形核白细胞(PMNL)和单个PMNL组分对伯氏疏螺旋体的杀灭作用。完整的PMNL杀死分贝。当螺旋体被特定的抗体调理时，伯氏杆菌的效率提高了6.5倍，完全提高了5倍。具有可激活的氧化酶的U-细胞质杀死了被激活的B。伯氏杆菌具有与完整的PMNL相似的杀灭未被调理的B的效率。勃格多费里。尽管B。伯格多菲对H_2O_2和NO敏感，PMNL裂解产物可杀死分贝。Burgdorferinini以及完整的PMNL杀死了OpsonizedB。伯氏杆菌可被PMNL抗菌成分弹性蛋白酶、LL-37、杀菌/通透性增加蛋白和人中性粒细胞多肽-1杀死。伯氏杆菌对溶菌酶的杀灭敏感性有限，不被天青杀菌素、蛋白酶3或乳铁蛋白杀死。有效地杀死了OFB。Burgdorferiby各种PMNL机制突出了螺旋体在体内矛盾的持久性。
The killing ofBorrelia burgdorferiby intact human polymorphonuclear leukocytes (PMNL) and by individual PMNL components was compared. Intact PMNL killedB. burgdorferi6.5-fold more efficiently and 5-fold more completely when spirochetes were opsonized with specific antibodies. U-cytoplasts, which have activatable oxidase, killed opsonizedB. burgdorferiwith an efficiency similar to that of intact PMNL in killing unopsonizedB. burgdorferi. AlthoughB. burgdorferiwere susceptible to H2O2and nitric oxide, PMNL lysates killedB. burgdorferinearly as well as intact PMNL killed opsonizedB. burgdorferi, suggesting a critical role for granule contents.B. burgdorferiwere killed by the PMNL antimicrobial components elastase, LL-37, bactericidal/permeability-increasing protein, and human neutrophil peptide-1.B. burgdorferihad limited susceptibility to killing by lysozyme and were not killed by azurocidin, proteinase 3, or lactoferrin. The efficient killing ofB. burgdorferiby a variety of PMNL mechanisms highlights the paradoxical persistence of spirochetes in vivo.