Increased contractility of isolated lung parenchyma in an animal model of pulmonary fibrosis induced by bleomycin.
Increased contractility of isolated lung parenchyma in an animal model of pulmonary fibrosis induced by bleomycin.
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博来霉素诱导的肺纤维化动物模型中离体肺实质的收缩性增加。
DOI：
10.1164/arrd.1982.125.1.89
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Adler,KB
中科院分区：
文献类型：
--
作者：
Evans,JN;Kelley,J;Low,RB;Adler,KB
将正常大鼠肺实质条带的收缩能力与气管内滴注硫酸博莱霉素诱导肺纤维化大鼠肺实质条带的收缩能力进行比较。随后，通过组织化学、免疫细胞化学和超微结构手段对每个条带中的收缩细胞群（肌肉和非肌肉）进行分析。响应于乙酰胆碱、肾上腺素和钾去极化溶液，纤维化条产生的力（克力/克组织湿重）大约是对照条的两倍。纤维化条带和对照条带中平滑肌的含量没有显着差异。免疫荧光检查表明，在组织学上缺乏平滑肌的区域，纤维化带增厚的肺泡壁中收缩蛋白（肌动蛋白和肌球蛋白）增加。纤维化间质的超微结构检查显示，富含丝的细胞数量增加，这些细胞似乎与收缩性间质细胞或肌成纤维细胞有关。结果表明，从纤维化大鼠肺中分离的实质条带可以在体外产生增加的力。这些反应可能与纤维化间质中非肌肉收缩细胞的增加有关。
The contractile capability off lung parenchymal strips isolated from normal rats was compared with that of strips isolated from rats with pulmonary fibrosis induced by intratracheal instillation of bleomycin sulfate. Subsequently, the population of contractile cells, both muscle and nonmuscle, was analyzed in each strip by histochemical, immunocytochemical, and ultrastructural means. The force (g force/g tissue wet weight) generated by fibrotic strips was approximately double that of the control strips in response to acetylcholine, epinephrine, and potassium depolarizing solution. The content of smooth muscle in fibrotic and control strips was not significantly different. Immunofluorescent examination indicated increased contractile proteins (actin and myosin) in the thickened alveolar walls of the fibrotic strips in areas devoid of histologically demonstrable smooth muscle. Ultrastructural examination of the fibrotic interstitium revealed an increased population of filament-laden cells that appeared related to the contractile interstitial cell, or myofibroblast. The results indicate that parenchymal strips isolated from fibrotic rat lung can generate increased forcein vitro. These responses may be related to increased nonmuscle contractile cells in the fibrotic interstitium.