Acute toxicity of cypermethrin on the juvenile of red claw crayfish Cherax quadricarinatus.
Acute toxicity of cypermethrin on the juvenile of red claw crayfish Cherax quadricarinatus.
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DOI：
10.1016/j.chemosphere.2019.124468
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发表时间：
2019-12
期刊：
影响因子：
8.8
通讯作者：
Julin Yuan;Jianlin Guo;Haiyang Wang;A. Guo;Qingping Lian;Z. Gu
中科院分区：
文献类型：
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作者：
Julin Yuan;Jianlin Guo;Haiyang Wang;A. Guo;Qingping Lian;Z. Gu
为了评价氯氰菊酯（Cypermethrin，简称氯氰菊酯）的毒性，测定了氯氰菊酯对红螯螯虾幼鱼的半数致死浓度（LC 50）。同时，分析了四氯化碳（0.1、1、10和100 ng·L-1）染毒96 h后，其肝胰腺抗氧化酶、生物转化酶和血清免疫酶的转录水平和含量，以揭示四氯化碳的毒性和解毒机制。24、48、72、96 h LC 50分别为1305.14、424.52、287.10和215.99 ng·L-1。在低浓度（0.16ng·L-1）时，酶的含量无显著变化。中浓度（0.32和0.63 ng·L-1）时，SOD和CAT含量在试验初期（24 h）迅速上升，后期下降;而高浓度（1.25ng·L-1）的甘草酸则显著抑制了SOD和CAT的含量。中、高浓度染毒72 h和96 h后，MDA、PC和GPx、EROD、CarE、GST含量均显著升高。Na+-K+-ATPase、PO、ALK含量在中、高浓度组均下降，以72 h和96 h组下降最为明显。转录改变类似于酶的内容，但转录反应一般比酶反应更直接。热休克蛋白（hsp 70）和多药耐药相关蛋白2（abcc 2）基因表达上调。
In order to assess the toxicity of Cypermethrin (CYP), the 50% lethal concentration (LC50) of CYP on the juvenile ofCherax quadricarinatusis assessed. Meanwhile, the transcription level and the content in the antioxidant and biotransformation enzymes in hepatopancreas and immune enzymes in the serum ofC. quadricarinatusexposed to CYP (0.1, 1, 10 and 100 ng·L-1) for 96 h were analyzed to reveal the CYP toxicity and detoxification mechanism. 24, 48, 72, 96 h LC50were 1305.14, 424.52, 287.10 and 215.99 ng·L-1, respectively. There was no significant change of the content of enzymes at low concentration (0.16 ng·L-1). The fast increase of SOD and CAT content was observed at early stage (24 h), subsequent decreased at later stage of trail at medium concentration (0.32 and 0.63 ng·L-1). However, high concentration (1.25 ng·L-1) of CYP significantly inhibited SOD and CAT content. There was a significant increase in the level of MDA, PC and the content of GPx, EROD, CarE, GST at medium and high concentration after 72 h and 96 h exposure. The Na+-K+-ATPase, PO, ALK content decreased at medium and high concentration, especially at the 72-h and the 96-h exposure. The transcription was altered similarly to enzyme content, but the transcriptional response was generally more immediate than enzymatic response. Heat shock protein (hsp70) and multidrug resistance-associated protein 2 (abcc2) genes were up-regulated.