Human Thy-1 (CD90) on activated endothelial cells is a counterreceptor for the leukocyte integrin Mac-1 (CD11b/CD18)
Human Thy-1 (CD90) on activated endothelial cells is a counterreceptor for the leukocyte integrin Mac-1 (CD11b/CD18)
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DOI：
10.4049/jimmunol.172.6.3850
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发表时间：
2004-03-15
影响因子：
4.4
通讯作者：
Saalbach, A
中科院分区：
文献类型：
--
作者：
Wetzel, A;Chavakis, T;Saalbach, A
响应炎症信号的白细胞募集部分是由属于Ig超家族的内皮细胞受体和白细胞整合素之间的相互作用控制的。在我们之前的工作中，人类Ig超家族糖蛋白Thy-1 （CD90）被鉴定为人类皮肤微血管内皮细胞上的激活相关细胞粘附分子。此外，Thy-1与单核细胞和多形核细胞上相应配体的相互作用被证明参与了这些白细胞与活化的表达Thy-1的内皮细胞的粘附。在这项研究中，我们已经确定了人类Thy-1和白细胞整合素Mac-1 (CD11b/CD18; α (M) β(2))在细胞系统和纯化形式中的特异性相互作用。单核细胞和多形核细胞可以粘附在表达人Thy-1的转染物上，也可以粘附在原代表达人真皮微血管内皮细胞上。此外，白细胞粘附到活化的内皮以及随后的跨内皮迁移是由Thy-1和Mac-1的相互作用介导的。白细胞-内皮相互作用的这一额外途径可能在白细胞向炎症部位募集的调节中发挥重要作用。
Leukocyte recruitment in response to inflammatory signals is in part governed by interactions between endothelial cell receptors belonging to the Ig superfamily and leukocyte integrins. In our previous work, the human Ig superfamily glycoprotein Thy-1 (CD90) was identified as an activation-associated cell adhesion molecule on human dermal microvascular endothelial cells. Furthermore, the interaction of Thy-1 with a corresponding ligand on monocytes and polymorphonuclear cells was shown to be involved in the adhesion of these leukocytes to activated Thy-1-expressing endothelial cells. In this study, we have identified the specific interaction between human Thy-1 and the leukocyte integrin Mac-1 (CD11b/CD18; alpha(M)beta(2)) both in cellular systems and in purified form. Monocytes and polymorphonuclear cells were shown to adhere to transfectants expressing human Thy-1 as well as to primary Thy-1-expressing human dermal microvascular endothelial cells. Furthermore, leukocyte adhesion to activated endothelium as well as the subsequent transendothelial migration was mediated by the interaction between Thy-1 and Mac-1. This additional pathway in leukocyte-endothelium interaction may play an important role in the regulation of leukocyte recruitment to sites of inflammation.