The CsrA-FliW network controls polar localization of the dual-function flagellin mRNA in Campylobacter jejuni.
The CsrA-FliW network controls polar localization of the dual-function flagellin mRNA in Campylobacter jejuni.
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DOI：
10.1038/ncomms11667
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发表时间：
2016-05-27
影响因子：
16.6
通讯作者：
Sharma CM
中科院分区：
文献类型：
--
作者：
Dugar G;Svensson SL;Bischler T;Wäldchen S;Reinhardt R;Sauer M;Sharma CM
广泛分布的CsrA/RsmA蛋白调节剂通过结合细菌mRNA中的GGA基序来抑制翻译。CsrA活性主要通过多个小调控RNA的螯合来控制。在这里，我们调查CsrA活性控制在没有拮抗小RNA的情况下，通过检查CsrA调节子在人类病原体空肠弯曲菌。我们使用全基因组免疫共沉淀结合RNA测序来表明CsrA主要结合鞭毛mRNA，并确定主要鞭毛蛋白mRNA（flaA）为CsrA的主要靶点。flaA mRNA被CsrA抑制，但它也可以滴定CsrA活性。与主C。空肠CsrA拮抗剂，FliW蛋白，flaA mRNA控制CsrA介导的其他鞭毛基因的转录后调节。RNA-FISH显示flaA mRNA表达并定位于伸长细胞的两极。极性flaA mRNA定位是翻译依赖性的，并且由CsrA-FliW网络进行转录后调节。总的来说，我们的研究结果表明CsrA-FliW在鞭毛组装和双功能mRNA定位的时空控制中的作用。 CsrA蛋白结合并抑制某些细菌mRNA的翻译。在这里，Dugar等人表明，对于人类病原体空肠弯曲杆菌，主要鞭毛蛋白mRNA既作为CsrA的靶标又作为调节“海绵”，并且以抑制依赖性方式定位于细胞极。
The widespread CsrA/RsmA protein regulators repress translation by binding GGA motifs in bacterial mRNAs. CsrA activity is primarily controlled through sequestration by multiple small regulatory RNAs. Here we investigate CsrA activity control in the absence of antagonizing small RNAs by examining the CsrA regulon in the human pathogen Campylobacter jejuni. We use genome-wide co-immunoprecipitation combined with RNA sequencing to show that CsrA primarily binds flagellar mRNAs and identify the major flagellin mRNA (flaA) as the main CsrA target. The flaA mRNA is translationally repressed by CsrA, but it can also titrate CsrA activity. Together with the main C. jejuni CsrA antagonist, the FliW protein, flaA mRNA controls CsrA-mediated post-transcriptional regulation of other flagellar genes. RNA-FISH reveals that flaA mRNA is expressed and localized at the poles of elongating cells. Polar flaA mRNA localization is translation dependent and is post-transcriptionally regulated by the CsrA-FliW network. Overall, our results suggest a role for CsrA-FliW in spatiotemporal control of flagella assembly and localization of a dual-function mRNA. The CsrA protein binds to and represses translation of certain bacterial mRNAs. Here, Dugar et al. show for the human pathogen Campylobacter jejuni that the major flagellin mRNA acts as both a target and a regulatory 'sponge' for CsrA, and is localized at the cell poles in a translation-dependent manner.