A novel proteoglycan from Ganoderma lucidum fruiting bodies protects kidney function and ameliorates diabetic nephropathy via its antioxidant activity in C57BL/6 db/db mice
A novel proteoglycan from Ganoderma lucidum fruiting bodies protects kidney function and ameliorates diabetic nephropathy via its antioxidant activity in C57BL/6 db/db mice
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灵芝子实体中的一种新型蛋白多糖通过其抗氧化活性保护 C57BL/6 db/db 小鼠的肾功能并改善糖尿病肾病
DOI：
10.1016/j.fct.2013.10.046
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发表时间：
2014-01-01
影响因子：
4.3
通讯作者：
Zhou, Ping
中科院分区：
文献类型：
--
作者：
Pan, Deng;Zhang, Dan;Zhou, Ping
糖尿病肾病（diabetic nephropathy，DN）是糖尿病患者发病的主要原因。因此，具有肾脏保护潜力的抗糖尿病药物将具有更高的治疗价值。从G.灵芝子实体对肾功能的影响。FYGL（250 mg/kg）不仅剂量依赖性地降低血糖浓度，（23.5%，p < 0.05），肾/体重比（23.6%，p < 0.01），血清肌酐（33.1%，p < 0.01），尿素氮（24.1%，P < 0.01），（35.9%，P < 0.01）和蛋白尿（30.7%，p < 0.01），但也增加了肾脏超氧化物歧化酶（75.3%，p < 0.01）、谷胱甘肽过氧化物酶（35.0%，p < 0.01）和过氧化氢酶活性（58.5%，p < 0.01）。FYGL治疗的DN小鼠的肾脏丙二醛含量（34.3%，p < 0.01）和8-羟基-2 '-脱氧鸟苷表达（2.5倍，p < 0.01）也较未治疗的DN小鼠降低，沿着肾脏形态学异常的改善。结论：FYGL通过增强抗氧化剂的活性和抑制氧化积累，对DN的肾功能和形态学损伤具有保护作用，提示FYGL是一种潜在的防治DN的营养补充剂。(C)2013爱思唯尔有限公司保留所有权利。
Diabetic nephropathy (DN) is the major cause of morbidity among diabetic patients. Thus, antidiabetic drugs with protection potential in the kidneys would have a higher therapeutic value. The effects of a novel proteoglycan, named FYGL, isolated from G. lucidum fruiting bodies, on the kidney function were investigated systematically in present work. FYGL (250 mg/kg) not only dosedependently reduced the blood glucose concentration (23.5%, p < 0.05), kidney/body weight ratio (23.6%, p < 0.01), serum creatinine (33.1%, p < 0.01), urea nitrogen (24.1%, p < 0.01),urea acid contents (35.9%, p < 0.01) and albuminuria (30.7%, p < 0.01)of DN mice compared to the untreated DN mice but also increased the renal superoxide dismutase (75.3%, p < 0.01), glutathione peroxidase (35.0%, p < 0.01) and catalase activities (58.5%, p < 0.01) compared to the untreated DN mice. The decreasing of renal malondialdehyde content (34.3%, p < 0.01) and 8-hydroxy-2'-deoxyguanosine expression (2.5-fold, p < 0.01) were also observed in FYGL-treated DN mice compared to the untreated DN mice, along with an amelioration of renal morphologic abnormalities. We conclude that FYGL confers protection against the renal functional and morphologic injuries by increasing activities of antioxidants and inhibiting accumulation of oxidation, suggesting a potential nutritional supplement for the prevention and therapy of DN. (C) 2013 Elsevier Ltd. All rights reserved.