Why are pathogenic staphylococci so lysozyme resistant?: The peptidoglycan O-acetyltransferase OatA is the major determinant for lysozyme resistance of Staphylococcus aureus
Why are pathogenic staphylococci so lysozyme resistant?: The peptidoglycan O-acetyltransferase OatA is the major determinant for lysozyme resistance of Staphylococcus aureus
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DOI：
10.1111/j.1365-2958.2004.04446.x
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发表时间：
2005-02-01
影响因子：
3.6
通讯作者：
Götz, F
中科院分区：
文献类型：
--
作者：
Bera, A;Herbert, S;Götz, F
葡萄球菌属属于少数对溶菌酶完全耐药的细菌属之一，这极大地有助于它们在人类和动物的皮肤及黏膜区域定殖的持久性和成功性。为了探究溶菌酶耐药的原因，我们在金黄色葡萄球菌中鉴定出一个基因oatA。相应的oatA缺失突变体对溶菌酶的敏感性增加。对细胞壁进行高效液相色谱和电喷雾电离串联质谱分析表明，野生型菌株的肽聚糖的胞壁酸在C6 - OH处被O - 乙酰化，而oatA突变体的胞壁酸缺乏这种修饰。互补的oatA突变体具有溶菌酶耐药性。我们在金黄色葡萄球菌中鉴定出首个细菌肽聚糖特异性O - 乙酰转移酶，并表明作为一种整合膜蛋白的OatA是葡萄球菌对溶菌酶高度耐药的分子基础。
Staphylococcus species belong to one of the few bacterial genera that are completely lysozyme resistant, which greatly contributes to their persistence and success in colonizing the skin and mucosal areas of humans and animals. In an attempt to discover the cause of lysozyme resistance, we identified a gene, oatA, in Staphylococcus aureus. The corresponding oatA deletion mutant had an increased sensitivity to lysozyme. HPLC and electrospray ionization tandem mass spectrometry analyses of the cell wall revealed that the muramic acid of peptidoglycan of the wild-type strain was O-acetylated at C6-OH, whereas the muramic acid of the oatA mutant lacked this modification. The complemented oatA mutant was lysozyme resistant. We identified the first bacterial peptidoglycan-specific O-acetyltransferase in S. aureus and showed that OatA, an integral membrane protein, is the molecular basis for the high lysozyme resistance in staphylococci.