Lipids in the inner membrane of dormant spores of Bacillus species are largely immobile
Lipids in the inner membrane of dormant spores of Bacillus species are largely immobile
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DOI：
10.1073/pnas.0306859101
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发表时间：
2004-05-18
影响因子：
11.1
通讯作者：
Setlow, P
中科院分区：
文献类型：
--
作者：
Cowan, AE;Olivastro, EM;Setlow, P
各种芽孢杆菌属物种的细菌孢子对许多容易穿透发芽孢子的化合物（包括甲胺）是不可渗透的或表现出低渗透性。我们现在表明，巨大芽孢杆菌和枯草芽孢杆菌的休眠孢子的内膜中的脂质探针在很大程度上是不动的，通过光漂白后的荧光再分布测量，但孢子萌发后变得自由扩散。脂质的不动性和缓慢渗透的甲胺通过内膜的休眠孢子可能是由于一个显着的（1.3- 1.6倍）明显减少的膜表面积在休眠孢子相对于萌发的孢子，但不是由于休眠孢子的高水平的吡啶二羧酸和二价阳离子。
Bacterial spores of various Bacillus species are impermeable or exhibit low permeability to many compounds that readily penetrate germinated spores, including methylamine. We now show that a lipid probe in the inner membrane of dormant spores of Bacillus megaterium and Bacillus subtilis is largely immobile, as measured by fluorescence redistribution after photobleaching, but becomes free to diffuse laterally upon spore germination. The lipid immobility in and the slow permeation of methylamine through the inner membrane of dormant spores may be due to a significant (1.3- to 1.6-fold) apparent reduction of the membrane surface area in the dormant spore relative to that in the germinated spore, but is not due to the dormant spore's high levels of dipicolinic acid and divalent cations.