Enhanced wound healing in diabetic rats by nanofibrous scaffolds mimicking the basketweave pattern of collagen fibrils in native skin
Enhanced wound healing in diabetic rats by nanofibrous scaffolds mimicking the basketweave pattern of collagen fibrils in native skin
复制标题
通过模仿天然皮肤中胶原纤维的篮状编织模式的纳米纤维支架增强糖尿病大鼠的伤口愈合
DOI：
10.1039/c7bm00545h
复制
发表时间：
2018-02-01
影响因子：
6.6
通讯作者：
Chen, Xiaofeng
中科院分区：
文献类型：
--
作者：
Sun, Luyao;Gao, Wendong;Chen, Xiaofeng
提供适当微环境线索以促进愈合过程的纳米纤维支架对于慢性伤口患者是非常理想的。虽然研究表明纤维组织在体外调节细胞行为，但对其在体内对伤口愈合过程的影响知之甚少。目前用于皮肤修复的大多数纳米纤维支架是随机取向的。在此，受天然皮肤中胶原纤维的编织图案的启发，我们通过静电纺丝制造具有交叉纤维组织的仿生纳米纤维支架。将交叉纳米纤维支架对成纤维细胞的调节与对齐和随机纳米纤维支架的调节进行比较。出乎意料的是，交叉的纳米纤维支架在成纤维细胞中诱导不同的细胞反应，包括与对齐或随机纳米纤维支架相比，细胞形态、迁移和伤口愈合相关基因表达的差异。更重要的是，在糖尿病大鼠模型中系统地研究了具有不同纤维组织的纳米纤维支架对伤口修复的调节作用。虽然所有纳米纤维支架都增强了愈合过程，但用交叉纳米纤维支架治疗的伤口获得了最佳愈合结果，这通过炎症的消退、成纤维细胞和角质形成细胞的加速迁移以及血管生成的促进来证明。这些发现有助于揭示纤维组织在体内调节伤口愈合过程中的作用，并表明仿生交叉纳米纤维支架用于修复慢性伤口的潜在效用。
Nanofibrous scaffolds that offer proper microenvironmental cues to promote the healing process are highly desirable for patients with chronic wounds. Although studies have shown that fiber organization regulates cell behaviors in vitro, little is known about its effects on the wound healing process in vivo. Most of the nanofibrous scaffolds currently used in skin repair are randomly oriented. Herein, inspired by the basketweave-like pattern of collagen fibrils in native skin, we fabricated biomimetic nanofibrous scaffolds with crossed fiber organization via electrospinning. The regulation of crossed nanofibrous scaffolds on fibroblasts was compared with that of aligned and random nanofibrous scaffolds. Unexpectedly, crossed nanofibrous scaffolds induced different cellular responses in fibroblasts, including differences in cellular morphology, migration and wound healing related gene expression, in comparison to either aligned or random nanofibrous scaffolds. More importantly, the regulation of nanofibrous scaffolds with different fiber organizations on wound repair was systematically investigated in diabetic rats. While the healing processes were enhanced by all nanofibrous scaffolds, wounds treated with crossed nanofibrous scaffolds achieved the best healing outcome, which was evidenced by the resolution of inflammation, the accelerated migration of fibroblasts and keratinocytes, and the promotion of angiogenesis. These findings helped reveal the role of fiber organization in regulating the wound healing process in vivo and suggest the potential utility of biomimetic crossed nanofibrous scaffolds for the repair of chronic wounds.