Functionalized semiconductor nanocrystals for ultrasensitive detection of peptides
Functionalized semiconductor nanocrystals for ultrasensitive detection of peptides
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DOI：
10.1016/j.aca.2005.03.071
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发表时间：
2005-06
影响因子：
6.2
通讯作者：
Xudong Chen;Xinbo Wang;Lin Liu;Dacheng Yang;Li Fan
中科院分区：
文献类型：
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作者：
Xudong Chen;Xinbo Wang;Lin Liu;Dacheng Yang;Li Fan
制备了半导体硫化镉纳米粒子，并用硫代钒酸对其进行了修饰。功能化的纳米颗粒是水溶性的。它们被用作荧光探针用于多肽的超灵敏检测。这种方法是基于在含有巯基的多肽(GN-9)的存在下功能化纳米镉的荧光增强，以及在多肽(GA-8和MT-25)的存在下功能化纳米镉的荧光猝灭。激发波长为360 nm，发射波长为530 nm。在最佳条件下，GN-9、GA-8和MT-25的校正曲线分别在0.15-3.5、0.2-4.0和0.2-3.8μ−1范围内呈线性关系。GN-9的检出限为0.010μ−1，GA-8的检出限为0.018μ−1，MT-25的检出限为0.022μ−1。该方法简便、快速、灵敏。
Semiconductor CdS nanoparticle have been prepared and modified with thiovanic acid. The functionalized nanoparticles are water-soluble. They were used as the fluorescence probes in the ultrasensitive detection of peptides. This method is based on the fluorescence enhancement of functionalized nano-CdS in the presence of peptide with mercapto groups (GN-9) and the fluorescence quenching of functionalized nano-CdS in the presence of peptide (GA-8 and MT-25). Excitation and emission wavelengths were 360 and 530nm, respectively. Under optimum conditions, the calibration graphs are linear over the range of 0.15–3.5, 0.2–4.0, and 0.2–3.8μgml−1for GN-9, GA-8 and MT-25, respectively. The corresponding detection limits were 0.010μgml−1for GN-9, 0.018μgml−1for GA-8 and 0.022μgml−1for MT-25, respectively. This method has been proved to be a simple, rapid and sensitive method.