Survivin expression correlates with apoptosis resistance after lymphocyte activation and is found preferentially in memory T cells
Survivin expression correlates with apoptosis resistance after lymphocyte activation and is found preferentially in memory T cells
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DOI：
10.1016/s0165-2478(01)00186-9
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发表时间：
2001-04-02
影响因子：
4.4
通讯作者：
Negrin, RS
中科院分区：
文献类型：
--
作者：
Kornacker, M;Verneris, MR;Negrin, RS
细胞凋亡的预防可能对免疫功能至关重要。Survivin是新近克隆的凋亡抑制蛋白家族成员。我们分析了生存素表达之前和之后的淋巴细胞活化分离的细胞群体。在激活之前，生存素是检测不到的。经IL-2和OKT-3激活后，CD 3（+）细胞表达Survivin。接下来，我们将生存素表达与培养物中随着时间的推移的Fas、Fast和细胞凋亡量相关。活化后，存活素在第2天即可被检测到，此后下降。在活化之前（第0天），Fas存在于60%的细胞上，并且到第2-6天存在于100%的细胞上。Fast mRNA表达峰值在第2天。在存活素表达高峰期（第2-4天），凋亡分数较低，但当存活素表达降低时，凋亡分数迅速增加。最后，我们发现活化后，CD 45 RO（+）记忆T细胞比CD 45 RA（+）初始T细胞表现出更高的生存素表达。这些结果表明，生存素可能有助于T细胞存活的早期T细胞反应，以及在记忆免疫反应。(C)2001 Elsevier Science B. V.保留所有权利。
The prevention of apoptosis may be critical for immunological function. Survivin is a recently cloned member of the inhibitor of apoptosis protein family. We analyzed survivin expression before and after lymphocyte activation in isolated cell populations. Prior to activation, survivin was undetectable. After activation with IL-2 and OKT-3, CD3(+) cells expressed survivin. Next, we correlated survivin expression with Fas, Fast and the amount of apoptosis over time in culture. After activation, survivin was readily detected by day 2 and decreased thereafter. Prior to activation (day 0), Fas was present on 60% of the cells and on 100% by days 2-6. Peak Fast mRNA expression was at day 2. During peak survivin expression (days 2-4) the apoptotic fraction was low, but when survivin expression decreased the apoptotic fraction increased rapidly. Finally, we found that CD45RO(+) memory T cells showed a higher expression of survivin than did CD45RA(+) naive T cells after activation. These results suggest that survivin may contribute to T-cell survival early in T-cell responses as well as in memory immune responses. (C) 2001 Elsevier Science B.V. All rights reserved.