IMPROVED AMPHOTERICIN-B ACTIVITY BY A MONOCLONAL ANTI-CRYPTOCOCCUS-NEOFORMANS ANTIBODY - STUDY DURING MURINE CRYPTOCOCCOSIS AND MECHANISMS OF ACTION
IMPROVED AMPHOTERICIN-B ACTIVITY BY A MONOCLONAL ANTI-CRYPTOCOCCUS-NEOFORMANS ANTIBODY - STUDY DURING MURINE CRYPTOCOCCOSIS AND MECHANISMS OF ACTION
复制标题
DOI：
10.1093/infdis/163.5.1114
复制
发表时间：
1991-05-01
影响因子：
6.4
通讯作者：
CHARREIRE, J
中科院分区：
文献类型：
--
作者：
DROMER, F;CHARREIRE, J
目前隐球菌病的治疗并不令人满意，特别是对艾滋病患者。采用DBA/2小鼠实验隐球菌病模型，研究小鼠单克隆抗隐球菌抗体（指定E1）是否能增强两性霉素B （AmB）的化疗作用。根据接种量的大小，这些小鼠自发死于急性肺炎（高接种量）或脑肿胀（低接种量）。与单独使用AmB相比，AmB和E1联合使用可显著提高两种模型小鼠的存活率。在体外研究了E1增强AmB活性的机制。当隐球菌与AmB或另一种多烯抗生素制霉菌素预孵育时，E1的结合增强。在E1或正常兔免疫球蛋白存在的情况下，AmB增强未刺激的腹腔巨噬细胞的吞噬作用。正常和免疫IgG有待进一步研究，以确定在何种情况下AmB能增强化疗效果。
Current therapy of cryptococcosis is unsatisfactory, particularly in patients with AIDS. Experimental cryptococcosis models in DBA/2 mice were used to determine whether the murine monoclonal anticryptococcal antibody (designated E1) might potentiate the chemotherapeutic effect of amphotericin B (AmB). According to the inoculum size, these mice died spontaneously from acute pneumonia (high inoculum) or from brain swelling (lower inoculum). AmB and E1 together significantly improved the survival of mice in both models compared with AmB alone. The mechanisms by which E1 might potentiate AmB activity were investigated in vitro. When cryptococci were preincubated with AmB or another polyene antibiotic, nystatin, there was an augmented binding of E1. AmB enhanced phagocytosis by unstimulated peritoneal macrophages in the presence of E1 or normal rabbit immunoglobulins. Normal and immune IgG deserve further study to determine under what circumstances the chemotherapeutic effect of AmB can be enhanced.