A Continuous Bovine Kidney Cell Line Constitutively Expressing Bovine αVβ6 Integrin Has Increased Susceptibility to Foot-and-Mouth Disease Virus
A Continuous Bovine Kidney Cell Line Constitutively Expressing Bovine αVβ6 Integrin Has Increased Susceptibility to Foot-and-Mouth Disease Virus
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DOI：
10.1128/jcm.03370-12
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发表时间：
2013-06-01
影响因子：
9.4
通讯作者：
Rodriguez, Luis L.
中科院分区：
文献类型：
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作者：
LaRocco, Michael;Krug, Peter W.;Rodriguez, Luis L.
口蹄疫（FMD）是一个世界性问题，限制了受影响国家的动物及其产品的贸易。从疾病暴发中快速分离、血清分型和疫苗匹配对于实施有效的疫苗接种计划和阻止暴发期间感染的传播至关重要。一些原代细胞已被证明对大多数口蹄疫病毒 (FMDV) 株高度敏感，但制备和维护困难且昂贵。由于α(V)β(6)整合素是FMDV的主要受体，因此我们转导牛肾细胞系以稳定表达α(V)和β(6)牛整合素亚基。该稳定细胞系 (LFBK-alpha(V)beta(6)) 在 >= 100 次细胞传代中表现出 beta(6) 表达，并且对 FMDV 感染的敏感性增强。 LFBK-alpha(V)beta(6) 细胞对于检测来自实验感染动物的所有血清型 FMDV 高度敏感，包括嗜猪型 FMDV 毒株 O/TAW/97。与目前用于病毒分离的其他细胞类型相比，LFBK-alpha(V)beta(6) 细胞在检测临床样本中的 FMDV 方面更有效，支持其作为更灵敏的病毒分离工具。
Foot-and-mouth disease (FMD) is a worldwide problem limiting the trade of animals and their products from affected countries. The rapid isolation, serotyping, and vaccine matching of FMD virus from disease outbreaks is critical for enabling the implementation of effective vaccination programs and to stop the spread of infection during outbreaks. Some primary cells have been shown to be highly susceptible to most strains of FMD virus (FMDV) but are difficult and expensive to prepare and maintain. Since the alpha(V)beta(6) integrin is a principal receptor for FMDV, we transduced a bovine kidney cell line to stably express both the alpha(V) and beta(6) bovine integrin subunits. This stable cell line (LFBK-alpha(V)beta(6)) showed beta(6) expression and enhanced susceptibility to FMDV infection for >= 100 cell passages. LFBK-alpha(V)beta(6) cells were highly sensitive for detecting all serotypes of FMDV from experimentally infected animals, including the porcinophilic FMDV strain O/TAW/97. In comparison to other cell types that are currently used for virus isolation, LFBK-alpha(V)beta(6) cells were more effective at detecting FMDV in clinical samples, supporting their use as a more sensitive tool for virus isolation.