Release of small transmitters through kiss-and-run fusion pores in rat pancreatic β cells
Release of small transmitters through kiss-and-run fusion pores in rat pancreatic β cells
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DOI：
10.1016/j.cmet.2006.08.011
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发表时间：
2006-10-01
期刊：
影响因子：
29
通讯作者：
Rorsman, Patrik
中科院分区：
文献类型：
--
作者：
MacDonald, Patrick E.;Braun, Matthias;Rorsman, Patrik
分泌囊泡的胞外分泌始于连接囊泡腔和细胞外间隙的融合孔。然后，这个孔可能会扩大或关闭，以完整地重新捕获囊泡。后者，被称为吻后跑，对胰腺P细胞大密核囊泡（ldcv）的胞吐作用的贡献是有争议的。单泡融合孔的检测表明，大鼠P细胞LDCVs可以通过快速的孔扩张、形成稳定的孔或通过短暂的小的吻跑融合孔进行胞吐。cAMP的升高使LDCV融合孔开口转变为瞬态模式。在这种条件下，小的融合孔足以释放ATP，与胰岛素一起储存在ldcv中。个人ATP释放事件与安培“独立脚”一致，代表亲吻和逃跑。因此，LDCV的“吻即跑”融合孔允许少量递质释放，但可能保留较大的胰岛素肽。这可能是选择性胰岛内信号传导的一种机制。
Exocytosis of secretory vesicles begins with a fusion pore connecting the vesicle lumen to the extracellular space. This pore may then expand or it may close to recapture the vesicle intact. The contribution of the latter, termed kiss-and-run, to exocytosis of pancreatic P cell large dense-core vesicles (LDCVs) is controversial. Examination of single vesicle fusion pores demonstrated that rat P cell LDCVs can undergo exocytosis by rapid pore expansion, by the formation of stable pores, or via small transient kiss-and-run fusion pores. Elevation of cAMP shifted LDCV fusion pore openings to the transient mode. Under this condition, the small fusion pores were sufficient for release of ATP, stored within LDCVs together with insulin. Individual ATP release events occurred coincident with amperometric "stand alone feet" representing kiss-and-run. Therefore, the LDCV kiss-and-run fusion pores allow small transmitter release but likely retain the larger insulin peptide. This may represent a mechanism for selective intraislet signaling.