Immunity in the absence of CD28 and CD137 (4-1BB) molecules.
Immunity in the absence of CD28 and CD137 (4-1BB) molecules.
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缺乏 CD28 和 CD137 (4-1BB) 分子的免疫。
DOI：
10.1046/j.1440-1711.2003.01153.x
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发表时间：
2003
影响因子：
4
通讯作者：
Kwon,ByoungS
中科院分区：
文献类型：
--
作者：
Vinay,DassS;Wolisi,GodwinO;Yu,Kang-Y;Choi,BeomK;Kwon,ByoungS
我们报告了CD 28和4 - 1BB（CD 137）基因缺陷小鼠的产生和免疫调节。这些小鼠存活，可生育，未显示任何明显异常，胸腺和脾脏中的T细胞表型正常。在4 - 1BB−/−中，抗CD 3和ConA的免疫应答增强，但在CD 28 −/−或双突变小鼠中均未增强，而在所有突变小鼠中，白细胞介素-2水平均降低。尽管4 - 1BB−/−小鼠显示大多数检测的免疫球蛋白同种型的基础水平增加，但与野生型对照相比，免疫球蛋白G2 a、免疫球蛋白G2和免疫球蛋白A的平台水平特别高。在4 - 1BB−/−小鼠中，免疫球蛋白类别转换为T依赖性抗原是正常的，但在CD 28 −/−和4 - 1BB−/− CD 28 −/−小鼠中受到极大影响。水泡性口炎病毒特异性细胞毒性T淋巴细胞应答和空斑减少中和能力在所有突变小鼠中均不同程度降低。对过敏原的接触敏感性在4 - 1BB−/−小鼠的耳厚度中显示出轻微但不显著的变化，但在CD 28 −/−和双突变小鼠中对相同抗原的接触超敏反应能力大大降低。
We report the generation and immune regulation of mice that are deficient in CD28 and 4‐1BB (CD137) genes. These mice were viable, fertile and did not display any overt abnormalities and had a normal T cell phenotype in thymus and spleen. Proliferative responses to anti‐CD3 and ConA were enhanced in 4‐1BB−/−but not in either CD28−/−or double mutant mice, while levels of interleukin‐2 were decreased in all mutant mice. Although the 4‐1BB−/−mice displayed increased basal levels of most immunoglobulin isotypes tested, the plateau levels of immunoglobulin G2a, immunoglobulin G2band immunoglobulin A were particularly high compared to wild type controls. The immunoglobulin class switch to T‐dependent antigen was normal in 4‐1BB−/−mice but was greatly affected in both CD28−/−and 4‐1BB−/−CD28−/−mice. Vesicular stomatitis virus‐specific cytotoxic T lymphocyte responses and plaque reduction neutralizing ability was differentially reduced in all mutant mice. Contact sensitivity to allergens showed marginal but not significant change in ear thickness in 4‐1BB−/−mice, but an ability to mount contact hypersensitivity to the same antigens was greatly curtailed in CD28−/−and double mutant mice.