Dual-radiolabelling of an injectable hyaluronan-tyramine-bisphosphonate hybrid gel for in vitro and in vivo tracking
Dual-radiolabelling of an injectable hyaluronan-tyramine-bisphosphonate hybrid gel for in vitro and in vivo tracking
复制标题
用于体外和体内追踪的可注射透明质酸-酪胺-二磷酸盐混合凝胶的双放射性标记
DOI：
10.1016/j.carbpol.2019.115652
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发表时间：
2020
影响因子：
11.2
通讯作者：
Yuchuan Yang
中科院分区：
文献类型：
--
作者：
Xia Yang;Jing Wang;Zhikai Ding;Qingchuan Lin;Liangang Zhuo;Wei Liao;Yan Zhao;Yue Feng;Yue Chen;Hongyuan Wei;Yuchuan Yang
透明质酸（HA）作为一种理想的生物材料得到了广泛的应用。了解它们的退化和分布对于更好地优化是很重要的。从使用放射性示踪剂的新角度出发，我们设计了ha -酪胺-双膦酸衍生物，用于同时用两种放射性核素（99mTc和131i）进行体外和体内跟踪。这种双放射性标记的透明质酸衍生物仍然可以与羟基磷灰石非共价交联形成可注射凝胶。凝胶的优良性能，如坚固，可生物降解和自愈能力得以保持。我们首先证明了使用高分辨率伽马射线能谱法区分降解凝胶中不同放射性核素的可能性。放射性标记的凝胶对多种细胞系的毒性低于纯羟基磷灰石，而他们的体内实验结果证明，凝胶的99mtc / 131i标记是安全稳定的，足以用于成像和定量跟踪。本方法也可用于其他多糖的双放射性标记凝胶的制备。
Hyaluronan (HA) have been widely used as the ideal biomaterials. It is important to understand their degradation and distribution for better optimization. From a new aspect of using radiotracers, we designed the HA-tyramine-bisphosphonate derivative for dual-labelling with two radionuclides (99mTc and131I) simultaneously forin vitroandin vivotracking. This dual-radiolabelled HA derivative can still be non-covalently crosslinked by hydroxyapatites to form injectable gel. The excellent properties of the gel, such as robust, biodegradable, and self-healing capacity were maintained. We firstly proved the possibility to distinguish different radionuclides in the degraded gel using the high-resolution gamma-ray spectrometry. The radiolabelled gel showed lower toxicity than pure hydroxyapatites against various cell lines, while thein vivoresults proved that the99mTc/131I-labelling of the gel was safe and stable enough for imaging and quantitatively tracking. The present method can also be applied for the development of dual-radiolabelled gels from other polysaccharides.