Long noncoding RNA THAP9-AS1 is induced by Helicobacter pylori and promotes cell growth and migration of gastric cancer
Long noncoding RNA THAP9-AS1 is induced by Helicobacter pylori and promotes cell growth and migration of gastric cancer
复制标题
长链非编码RNA THAP9-AS1由幽门螺杆菌诱导并促进胃癌细胞生长和迁移
DOI：
10.2147/ott.s201832
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发表时间：
2019-01-01
影响因子：
4
通讯作者：
Li, Wenjuan
中科院分区：
文献类型：
--
作者：
Jia, Wenxiao;Zhang, Jiaqin;Li, Wenjuan
研究背景长链非编码RNA（LncRNA）已被证实在肿瘤生物学中发挥重要作用。胃癌（GC）是第三大癌症相关死亡原因，幽门螺杆菌（H. pylori）是胃癌的主要危险因素。在本研究中，我们重点研究了H。幽门螺杆菌相关lncRNA在胃癌进展中的作用方法采用RNA测序技术对H.幽门感染的GC细胞。结果胃癌细胞感染H. pylori的表达，胃癌组织高于胃炎组织。集落形成实验、CCK 8实验和transwell实验表明THAP 9-AS 1能促进胃癌细胞的增殖和迁移。我们的研究鉴定了原癌lncRNA THAP 9-AS 1，其在GC组织中的表达水平高于在胃炎组织中的表达水平，并且其在体外促进GC细胞的增殖和迁移。结论本研究为H.幽门相关性胃癌
Background Long noncoding RNAs (LncRNAs) have been confirmed to play crucial roles in cancer biology. Gastric cancer (GC) is the third leading cause of cancer related death, and Helicobacter pylori (H. pylori) is the major risk factor for GC. In this study, we focused on the roles of H. pylori-related lncRNAs in the progression of GC. Method Differentially expressed lncRNAs were identified through RNA-seq analysis of H. pylori-infected GC cells. Results We found that the expression of the lncRNA THAP9-AS1 was up-regulated after infection of GC cells with H. pylori and was higher in GC tissues than in gastritis tissues. Colony formation, CCK8 and transwell assays were executed to show that THAP9-AS1 can promote GC cell proliferation and migration in vitro. Our study identified the pro-oncogenic lncRNA THAP9-AS1, which has a higher expression level in GC tissues than in gastritis tissues and which promoted the proliferation and migration of GC cells in vitro. Conclusion These findings may provide a potential therapeutic target for H. pylori-associated GC.