Expression of the 37-kDa laminin binding protein in murine lung tumor cell correlates with tumor angiogenesis.
Expression of the 37-kDa laminin binding protein in murine lung tumor cell correlates with tumor angiogenesis.
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DOI：
10.1016/s0304-3835(00)00365-7
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发表时间：
2000-05
期刊：
影响因子：
9.7
通讯作者：
M. Tanaka;K. Narumi;M. Isemura;M. Abe;Y. Sato;T. Abe;Y. Saijo;T. Nukiwa;K. Satoh
中科院分区：
文献类型：
--
作者：
M. Tanaka;K. Narumi;M. Isemura;M. Abe;Y. Sato;T. Abe;Y. Saijo;T. Nukiwa;K. Satoh
37-kDa层粘连蛋白结合蛋白（37 LBP）（67-kDa层粘连蛋白受体的前体蛋白）的表达与癌细胞的生物侵袭性密切相关。以前，我们已经建立了小鼠肺癌细胞系T11和T15，其中37 LBP的表达显着减少，并报告说，T11和T15受体的平均生存时间显着延长与对照细胞系（P29和T42）相比。免疫组化结果显示，T11和T15肿瘤的微血管密度分别为28.1±7.2/mm ~ 2和29.7±6.5/mm ~ 2，显著低于P29和T42肿瘤的微血管密度（分别为46.3±8.7/mm ~ 2和50.5±4.4/mm ~ 2）。与P29和T42中的表达相比，T11和T15中的血管内皮生长因子（VEGF）表达受到抑制。T11和T15细胞的条件培养基显示毛细血管内皮细胞的增殖和迁移显著降低。这些结果表明，37 LBP在反义RNA转染子中的表达降低可能与其低致瘤性有关，这种作用可能部分是由于小鼠肺癌的肿瘤血管生成减少所致。
Expression of the 37-kDa laminin binding protein (37LBP), a precursor protein of the 67-kDa laminin receptor, correlates well with the biological aggressiveness of cancer cells. Previously, we have established murine lung cancer cell lines T11 and T15, in which 37LBP expression was remarkably diminished, and reported that the mean survival time of the T11 and the T15-recipients was significantly prolonged compared with that of the control cell lines (P29 and T42). In the present study, immunohistochemical findings of the tumors demonstrated that the microvessel density in the T11 (28.1±7.2/mm2) and in the T15 tumor (29.7±6.5/mm2) were significantly lower than that observed in P29 (46.3±8.7/mm2) or in T42 (50.5±4.4/mm2). Expression of vascular endothelial growth factor (VEGF) was repressed in T11 and T15 compared with its expression in P29 and T42. It was also shown that conditioned media of T11 and T15 cells exhibited significantly reduced proliferation and migration of the capillary endothelial cells. These results suggest that decreased expression of 37LBP in antisense-RNA transfectant may relate to its low tumorigenicity, and that this effect may be partly caused by the diminished tumor angiogenesis of murine lung cancer.