Expression and clinical significance of long non-coding RNA HNF1A-AS1 in human gastric cancer.
Expression and clinical significance of long non-coding RNA HNF1A-AS1 in human gastric cancer.
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DOI：
10.1186/s12957-015-0706-3
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发表时间：
2015-10-15
影响因子：
3.2
通讯作者：
Huang Q
中科院分区：
文献类型：
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作者：
Dang Y;Lan F;Ouyang X;Wang K;Lin Y;Yu Y;Wang L;Wang Y;Huang Q
越来越多的证据表明，长链非编码RNA（lncRNA）在包括胃癌（GC）在内的人类肿瘤的发生、发展中起着重要作用。然而，lncRNA的功能和临床意义仍然知之甚少。本研究首先采用lncRNA微阵列技术检测了6例胃癌组织中LncRNA HNF 1A反义RNA 1（HNF 1A-AS 1）的表达，然后采用实时荧光定量逆转录PCR（qRT-PCR）技术进一步检测了3株胃癌细胞系和161例胃癌组织中HNF 1A-AS 1的表达。我们还评估了HNF 1A-AS 1表达与胃癌患者临床病理特征之间的关系。LncRNA微阵列分析结果显示，HNF 1A-AS 1在GC组织中下调（平均倍数变化2.06，p < 0.05），qRT-PCR进一步证实了这一点。qRT-PCR结果显示，HNF 1A-AS 1在3个胃癌细胞系（AGS、BGC-823和MKN-45）中的表达不仅相对于正常胃粘膜上皮细胞系（GES-1）下调，而且相对于配对的相邻非肿瘤组织，在胃癌组织中也下调（低表达，161例中有94例;低表达率，58.38%）。此外，低HNF 1A-AS 1表达与肿瘤大小/直径（p = 0.005，多变量分析）、血清癌胚抗原（CEA）和碳水化合物抗原19-9（CA 19 -9）水平以及组织样本中RRM 1表达相关（分别为p = 0.028、p = 0.009和p = 0.006）。总之，我们的数据表明lncRNA HNF 1A-AS 1可能是GC的调节因子，因此，它可能具有作为这种类型癌症的新生物标志物和治疗靶点的潜力。
Increasing evidence has demonstrated that long non-coding RNAs (lncRNAs) play essential roles in the occurrence and development of human cancers, including gastric cancer (GC). However, the functional and clinical significance of lncRNAs are still poorly understood. In this study, the expression of LncRNA HNF1A antisense RNA 1 (HNF1A-AS1) was first examined by lncRNAs microarray analysis in 6 GC tissues, and was then further verified by real-time quantitative reverse transcription PCR (qRT-PCR) both in 3 GC cell lines and 161 cases of GC tissues. We also evaluated the association between HNF1A-AS1 expression and clinicopathological features of patients with GC. LncRNAs microarray analysis results exhibited that HNF1A-AS1 was downregulated in GCs tissues (mean fold change 2.06, p < 0.05), which was further confirmed by qRT-PCR. The results from qRT-PCR showed that the expression of HNF1A-AS1 was not only downregulated in three GC cell lines (AGS, BGC-823, and MKN-45) relative to that in a normal gastric mucosal epithelial cell line (GES-1), but also decreased in GC tissues relative to that in paired adjacent non-neoplastic tissues (low expression, 94 of 161; low expression rate, 58.38 %). Furthermore, low HNF1A-AS1 expression was associated with tumor size/diameter (p = 0.005, multivariate analysis), levels of serum carcinoembryonic antigen (CEA), and carbohydrate antigen 19-9 (CA19-9), and RRM1 expression in tissue samples (p = 0.028, p = 0.009, and p = 0.006, respectively). Taken together, our data indicate that lncRNA HNF1A-AS1 may be a regulator of GC, and thus, it may have potential as a novel biomarker and treatment target for this type of cancer.