Association Between Environmental Microbiota and Indigenous Bacteria Found in Hemolymph, Extrapallial Fluid and Mucus of Anodonta cygnea (Linnaeus, 1758)
Association Between Environmental Microbiota and Indigenous Bacteria Found in Hemolymph, Extrapallial Fluid and Mucus of Anodonta cygnea (Linnaeus, 1758)
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DOI：
10.1007/s00248-010-9649-y
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发表时间：
2010-08-01
影响因子：
3.6
通讯作者：
da Costa, Paulo Martins
中科院分区：
文献类型：
--
作者：
Antunes, Filipa;Hinzmann, Mariana;da Costa, Paulo Martins
双壳贝类过滤并积累大量存在于采集水中的微生物。对小天鹅无齿蚌与其周围环境中存在的微生物群之间的平衡的完整理解仍然不完整。因此，本研究的目的是定量和鉴定的土著细菌的生物流体中的A。采自葡萄牙北方米拉泻湖的小天鹅。结果表明，梅氏弧菌和温和气单胞菌为优势菌群。粘液中细菌总数的中位数为3.1 x 10（3）CFU g（-1），而液体中的平均值范围为1.5 x 10（2）至6.5 x 10（2）CFU ml（-1）。在实验过程中，未在健康人A.小天鹅。然而，E.大肠杆菌和肠球菌在米拉泻湖揭示了其存在于水中。我们的观察表明，A. cygnea对E.大肠杆菌，存在于周围环境中，通过由血淋巴循环细胞（血细胞）进行的主动吞噬过程。
Bivalves filter and accumulate large numbers of microorganisms present in the harvesting water. A complete understanding of the balance between Anodonta cygnea and the microbiota present in their surrounding environment remains incomplete. Therefore, the aim of this study was to quantify and identify the indigenous bacteria in the biological fluids of A. cygnea collected from Mira Lagoon in northern Portugal. The results showed Vibrio metschnikovii and Aeromonas sobria as the dominant groups. The median for total bacteria from mucus was 3.1 x 10(3) CFU g(-1), whereas the range in means from fluids was 1.5 x 10(2) to 6.5 x 10(2) CFU ml(-1). During the experimental work, Escherichia coli and enterococci were not detected in healthy A. cygnea. However, the periodic detection of E. coli and enterococci in Mira lagoon revealed its presence in the water. Our observations suggest that A. cygnea has the ability to filter and eliminate E. coli, present in the surrounding environment, through an active phagocytic process conducted by hemolymph circulating cells, the hemocytes.