Non-contact photothermal control of enzyme reactions on a microchip by using a compact diode laser
Non-contact photothermal control of enzyme reactions on a microchip by using a compact diode laser
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DOI：
10.1016/s0021-9673(00)00593-8
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发表时间：
2000-10-13
影响因子：
4.1
通讯作者：
Kitamori, T
中科院分区：
文献类型：
--
作者：
Tanaka, Y;Slyadnev, MN;Kitamori, T
利用半导体激光器实现了微芯片中酶催化反应的光热温度控制。使用能量为10 mW的激光束照射放置在微芯片盖板顶部的吸收目标。理论计算表明，由于靶释放的热量，微通道中的温度可以在短时间间隔内局部升高5-7摄氏度。酶反应的速率，这是最初抑制由于芯片冷却到低温，增加了当目标被照射。产物用热透镜显微镜检测。结果表明，产物浓度依赖于照射时间，激光强度和基板浓度。然后从这些依赖性中推导出反应特性（反应的速率常数）。反应体积和反应产物的绝对量分别估计为10 nl和100 fmol。研究还表明，使用红外辐射的直接溶剂加热方法可以控制微通道中的反应。(C)2000 Elsevier Science B. V.保留所有权利。
Photothermal temperature control of an enzyme-catalyzed reaction in a microchip using a diode laser was demonstrated. A laser beam with energy of 10 mW was used to irradiate an absorbing target placed on top of the microchip cover plate. Theoretical calculations have shown that temperature in the microchannel can be locally increased by 5-7 degrees C during short time intervals, due to heat released by the target. The rate of the enzyme reaction, which was initially inhibited due to cooling of the chip to low temperature, was increased when the target was irradiated. The products were detected by a thermal lens microscope. The product concentration was shown to depend on irradiation time, laser intensity and substrate concentration. Reaction characteristics (rate constant of the reaction) were then derived from these dependencies. The reaction volume and absolute quantity of the reaction product were estimated as 10 nl and 100 fmol, respectively. It was also demonstrated that a direct solvent heating method using infrared radiation could control the reaction in the microchannel. (C) 2000 Elsevier Science B.V. All rights reserved.