Xanthomonas citri subsp citri surface proteome by 2D-DIGE: Ferric enterobactin receptor and other outer membrane proteins potentially involved in citric host interaction
Xanthomonas citri subsp citri surface proteome by 2D-DIGE: Ferric enterobactin receptor and other outer membrane proteins potentially involved in citric host interaction
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DOI：
10.1016/j.jprot.2016.05.007
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发表时间：
2017-01-16
影响因子：
3.3
通讯作者：
Marques Novo-Mansur, Maria Teresa
中科院分区：
文献类型：
--
作者：
Carnielli, Carolina Moretto;Artier, Juliana;Marques Novo-Mansur, Maria Teresa
柑橘黄单胞菌亚种citri (XAC) 是柑橘溃疡病的病原体，柑橘溃疡病是一种对世界各地经济影响巨大的疾病。了解蛋白质在 XAC 细胞表面的作用可以为病原体与植物相互作用提供新的见解。通过标记完整细胞，然后进行细胞裂解和直接 2D-DIGE 分析，在体内（感染性）和体外（非感染性）条件下生长的 XAC 中进行表面蛋白质组分析。通过质谱法分析了七十九个差异点。体内条件下，含有 Dnal( 蛋白、60 kDa 伴侣蛋白、保守假设蛋白、苹果酸脱氢酶、磷酸甘露糖异构酶和铁肠杆菌素受体的斑点观察到最高相对丰度。在斑点中发现延伸因子 Tu、OmpA 相关蛋白、Oar 蛋白和一些 Ton-B 依赖性受体在体内减少。一些鉴定出的蛋白 在感染条件下，XAC 表面上存在并预测为细胞质的分子，例如 DnaK 和 60 KDa 伴侣蛋白，此前也曾在其他微生物的细胞表面发现过。这是对 XAC 表面蛋白质组的首次研究，结果表明分子伴侣在 XAC-柑橘相互作用中起到调节作用。这里使用的方法可以应用于其他病原体-宿主相互作用系统，并有助于获得新的见解 细菌对生物技术感兴趣的有希望的目标的致病性。生物学意义：这项研究为黄单胞菌的当前知识提供了新的见解。致病性。 2D-DIGE 方法首次应用于完整细胞，以寻找参与病原体与植物相互作用的表面蛋白。结果表明新的表面/外膜蛋白参与 XAC 与其柑橘宿主之间的相互作用，并且可以 为柑橘溃疡病控制提供生物技术兴趣的潜在目标。 (C) 2016 Elsevier B.V. 保留所有权利。
Xanthomonas citri subsp. citri (XAC) is the causative agent of citrus canker, a disease of great economic impact around the world. Understanding the role of proteins on XAC cellular surface can provide new insights on pathogen-plant interaction. Surface proteome was performed in XAC grown in vivo (infectious) and in vitro (non-infectious) conditions, by labeling intact cells followed by cellular lysis and direct 2D-DIGE analysis. Seventy-nine differential spots were analyzed by mass spectrometry. Highest relative abundance for in vivo condition was observed for spots containing Dnal(protein, 60 kDa chaperonin, conserved hypothetical proteins, malate dehydrogenase, phosphomannose isomerase, and ferric enterobactin receptors. Elongation factor Tu, OmpA-related proteins, Oar proteins and some Ton-B dependent receptors were found in spots decreased in vivo. Some proteins identified on XAC's surface in infectious condition and predicted to be cytoplasmic, such as DnaK and 60 KDa chaperonin, have also been previously found at cellular surface in other microorganisms. This is the first study on XAC surface proteome and results point to mediation of molecular chaperones in XAC-citrus interaction. The approach utilized here can be applied to other pathogen-host interaction systems and help to achieve new insights in bacterial pathogenicity toward promising targets of biotechnological interest.Biological significance: This research provides new insights for current knowledge of the Xanthomonas sp. pathogenicity. For the first time the 2D-DIGE approach was applied on intact cells to find surface proteins involved in the pathogen-plant interaction. Results point to the involvement of new surface/outer membrane proteins in the interaction between XAC and its citrus host and can provide potential targets of biotechnological interest for citrus canker control. (C) 2016 Elsevier B.V. All rights reserved.