The Protein Corona of Plant Virus Nanoparticles Influences their Dispersion Properties, Cellular Interactions, and In Vivo Fates.
The Protein Corona of Plant Virus Nanoparticles Influences their Dispersion Properties, Cellular Interactions, and In Vivo Fates.
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DOI：
10.1002/smll.201502458
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发表时间：
2016-04-06
期刊：
影响因子：
13.3
通讯作者：
Steinmetz, Nicole F.
中科院分区：
文献类型：
--
作者：
Pitek, Andrzej S.;Wen, Amy M.;Shukla, Sourabh;Steinmetz, Nicole F.
体液如血浆中的生物分子可以吸附到纳米颗粒的表面并影响其生物学特性。这种现象被称为蛋白质冠，在合成纳米技术领域已经建立，但尚未在植物病毒纳米颗粒（VNPs）的背景下描述。我们研究了来自烟草花叶病毒（TMV）和血浆蛋白的VNP之间的相互作用，发现VNP蛋白质冠是显着丰富的合成颗粒的冠相比。形成的冠主要由补体蛋白和免疫球蛋白，其结合可以通过PEG化VNP表面来减少。我们研究了VNP蛋白冠在癌症和血栓形成背景下对分子识别和细胞靶向的影响。具有PEG和靶向整联蛋白或纤维蛋白（原）的肽配体的功能化TMV棒的文库显示出不同的分散性质、细胞相互作用和体内命运，这取决于蛋白质冠的性质，影响靶特异性和巨噬细胞的非特异性清除。我们的研究结果提供了深入了解VNPs的体内特性，并建议在开发有效的靶向VNP制剂期间应考虑蛋白质电晕效应。研究了靶向植物病毒纳米颗粒（VNPs）与血浆蛋白之间的相互作用及其后果。与原位VNPs相关的蛋白质的量低于合成纳米颗粒;补体蛋白和免疫球蛋白占主导地位。蛋白质吸附的分子靶向和体内生物分布的后果进行了证明。
Biomolecules in bodily fluids such as plasma can adsorb to the surface of nanoparticles and influence their biological properties. This phenomenon, known as the protein corona, is well established in the field of synthetic nanotechnology but has not been described in the context of plant virus nanoparticles (VNPs). We investigated the interaction between VNPs derived from Tobacco mosaic virus (TMV) and plasma proteins, and found that the VNP protein corona was significantly less abundant compared to the corona of synthetic particles. The formed corona was dominated by complement proteins and immunoglobulins, the binding of which could be reduced by PEGylating the VNP surface. We investigated the impact of the VNP protein corona on molecular recognition and cell targeting in the context of cancer and thrombosis. A library of functionalized TMV rods with PEG and peptide ligands targeting integrins or fibrin(ogen) showed different dispersion properties, cellular interactions and in vivo fates depending on the properties of the protein corona, influencing target specificity and non-specific scavenging by macrophages. Our results provide insight into the in vivo properties of VNPs and suggest that the protein corona effect should be considered during the development of efficacious, targeted VNP formulations. The interactions between targeted plant virus-based nanoparticles (VNPs) and blood plasma proteins and their consequences are investigated. The amount of proteins associated with VNPs in situ is lower than for synthetic nanoparticles; complement proteins and immunoglobulins dominate. The consequences of protein adsorption for molecular targeting and in vivo biodistribution are demonstrated.
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影响因子：
13.8
作者：
Godin B;Sakamoto JH;Serda RE;Grattoni A;Bouamrani A;Ferrari M
通讯作者：
Ferrari M
影响因子：
4.7
作者：
Chariou PL;Lee KL;Wen AM;Gulati NM;Stewart PL;Steinmetz NF
通讯作者：
Steinmetz NF
影响因子：
20.3
作者：
Agemy, Lilach;Sugahara, Kazuki N.;Ruoslahti, Erkki
通讯作者：
Ruoslahti, Erkki
影响因子：
4.4
作者：
Dobrov, Evgeny N.;Nikitin, Nikolai A.;Atabekov, Joseph G.
通讯作者：
Atabekov, Joseph G.
影响因子：
4.7
作者：
McCarthy JR;Patel P;Botnaru I;Haghayeghi P;Weissleder R;Jaffer FA
通讯作者：
Jaffer FA