A guideline for sample preparation in modern tree-ring stable isotope research
A guideline for sample preparation in modern tree-ring stable isotope research
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DOI：
10.1016/j.dendro.2017.05.002
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发表时间：
2017-06-01
影响因子：
3
通讯作者：
Helle, Gerhard
中科院分区：
文献类型：
--
作者：
Schollaen, Karina;Baschek, Heiko;Helle, Gerhard
木材样品的制备包括树轮解剖、纤维素提取、均质和包装以进行稳定同位素分析，这是一个费时费力的过程。在简要总结现有方法的基础上，提出了一种从分析前的考虑到木材样品制备、从树木年轮截面半自动化学提取纤维素到用于稳定同位素比质谱分析的树木年轮解剖的方法学方法：横截面提取和解剖(CSED)指南。遵循CSED指南可以大大提高树轮稳定同位素测量的效率，与经典方法相比，我们介绍了一种用户友好的纤维素提取设备，允许同时处理总长180厘米(相当于6个30厘米长的增量芯)和厚度0.6-2.0 mm的木材横截面。经过纤维素提取后，具有不同木材生长速率和树轮边界的10个树种(针叶木和被子植物木材)的树轮结构基本保持良好的可识别性。此外，我们证明了利用简单的人工设备和复杂的紫外光激光解剖显微镜，可以从年度到季节内的分辨率解剖来自纤维素横截面的树轮，在大多数情况下不再需要样品均化。
The comprehensive procedure of wood sample preparation, including tree-ring dissection, cellulose extraction, homogenization and packing for stable isotope analysis, is labour intensive and time consuming.Based on a brief compilation of existing methods, we present a methodological approach from pre-analyses considerations to wood sample preparation, semi-automated chemical extraction of cellulose from tree-ring cross-sections, and tree-ring dissection for stable isotope ratio mass spectrometry: the Cross-Section Extraction and Dissection (CSED) guideline. Following the CSED guideline can considerably increase efficiency of tree-ring stable isotope measurement compared to classical methods We introduce a user-friendly device for cellulose extraction, allowing simultaneous treatment of wood cross-sections of a total length of 180 cm (equivalent to 6 increment cores of 30 cm length) and thickness of 0.6-2.0 mm. After cellulose extraction, tree-ring structures of 10 tree species (coniferous and angiosperm wood) with different wood growth rates and tree-ring boundaries, largely remained well identifiable.Further, we demonstrate that tree rings from cellulose cross-sections can be dissected at annual to intra-seasonal resolution, utilizing simple manual devices as well as sophisticated UV-laser microdissection microscopes in a way that sample homogenization is no longer necessary in most cases.We investigate seasonal precipitation signals in high-resolution intra-annual delta O-18 cellulose values from African baobab, performed by using UV-laser microdissection microscopes.