MECHANISM FOR REGULATING CELL-SURFACE DISTRIBUTION OF NA+, K+-ATPASE IN POLARIZED EPITHELIAL-CELLS
MECHANISM FOR REGULATING CELL-SURFACE DISTRIBUTION OF NA+, K+-ATPASE IN POLARIZED EPITHELIAL-CELLS
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DOI：
10.1126/science.1658934
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发表时间：
1991-11-08
期刊：
影响因子：
56.9
通讯作者：
NELSON, WJ
中科院分区：
文献类型：
--
作者：
HAMMERTON, RW;KRZEMINSKI, KA;NELSON, WJ
钠、钾腺苷三磷酸酶（Na+，K+-ATPase）限制于极化上皮细胞的顶膜或基底-侧膜区域是许多组织和器官中离子和溶质转运的基础。实验发现，Madin-Darby犬肾（MDCK）上皮细胞Na+，K+-ATP酶在膜上的限制性分布是由于活性酶优先保留在基底-侧膜区，而选择性失活和丢失在顶膜区，而不是由高尔基复合体合成的新蛋白质定向到基底-侧膜区。这些结果表明，在正常极化上皮细胞和疾病状态下，Na+，K+-ATP酶的相同亚基的不同分布可能会产生。
Restriction of sodium, potassium adenosine triphosphatase (Na+, K+-ATPase) to either the apical or basal-lateral membrane domain of polarized epithelial cells is fundamental to vectorial ion and solute transport in many tissues and organs. A restricted membrane distribution of Na+, K+-ATPase in Madin-Darby canine kidney (MDCK) epithelial cells was found experimentally to be generated by preferential retention of active enzyme in the basal-lateral membrane domain and selective inactivation and loss from the apical membrane domain, rather than by vectorial targeting of newly synthesized protein from the Golgi complex to the basal-lateral membrane domain. These results show how different distributions of the same subunits of Na+, K+-ATPase may be generated in normal polarized epithelia and in disease states.