Histone Acetylation Induced Transformation of B-DNA to Z-DNA in Cells Probed through FT-IR Spectroscopy
Histone Acetylation Induced Transformation of B-DNA to Z-DNA in Cells Probed through FT-IR Spectroscopy
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通过 FT-IR 光谱探测细胞中组蛋白乙酰化诱导 B-DNA 向 Z-DNA 的转化
DOI：
10.1021/acs.analchem.6b00400
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发表时间：
2016
影响因子：
7.4
通讯作者：
Chen Zhu
中科院分区：
文献类型：
--
作者：
Zhang Fengqiu;Huang Qing;Yan Jingwen;Chen Zhu
核小体由 DNA 和组蛋白组成，组蛋白的乙酰化会扰乱 DNA 和组蛋白的相互作用，从而影响染色质构象和功能。然而，乙酰化是否或如何诱导 DNA 构象变化仍然难以捉摸。在这项工作中，我们应用 FT-IR 光谱来监测细胞中的 DNA 信号，因为组蛋白乙酰化受到曲古抑菌素 A (TSA)（一种组蛋白脱乙酰酶 (HDAC) 的可逆抑制剂）的调节。我们的结果明确证明，在 TSA 处理的 HeLa 细胞中，组蛋白乙酰化后 B-DNA 显着转变为 Z-DNA。这是第一份为细胞表观遗传状态下 DNA 的 B-Z 转化提供明确实验证据的报告。
A nucleosome is made up of DNA and histones, and acetylation of histones perturbs the interaction of DNA and histones and thus affects the chromatin conformation and function. However, whether or how acetylation induces DNA conformation changes is still elusive. In this work, we applied FT-IR spectroscopy to monitor the DNA signals in cells as the histone acetylation was regulated by trichostatin A (TSA), a reversible inhibitor to histone deacetylases (HDACs). Our results unambiguously demonstrate the significant transformation of B-DNA to Z-DNA upon histone acetylation in the TSA treated HeLa cells. This is the first report providing the explicit experimental evidence for such a B-Z transformation of DNA in the epigenetic states of cells.