Tamoxifen Increases Membrane Fluidity at High Concentrations
Tamoxifen Increases Membrane Fluidity at High Concentrations
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高浓度他莫昔芬可增加膜流动性
DOI：
10.1023/a:1005515502954
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发表时间：
2000
影响因子：
4
通讯作者：
F. Zorlu
中科院分区：
文献类型：
--
作者：
F. Severcan;N. Kazanci;F. Zorlu
关于他莫昔芬对膜流动性的影响，文献中存在相互矛盾的结果。目前的工作研究了他莫昔芬对膜动力学的影响，以查明他莫昔芬的浓度是否是造成这种差异的因素之一。浊度（440 nm 处的吸光度）和傅里叶变换红外光谱研究表明，他莫昔芬在低浓度（1 mol.%）和高浓度（30 mol.%）他莫昔芬时对膜流动性产生相反的影响。低他莫昔芬浓度增加凝胶和液晶相的吸光度，而高他莫昔芬浓度降低凝胶和液晶相的吸光度，而他莫昔芬浓度降低吸光度。对两个相的观察表明，CH2 伸缩带的带宽随着 1 mol.% 他莫昔芬的存在而减小，并随着 30 mol.% 他莫昔芬的存在而增加，表明在低他莫昔芬浓度下膜流动性降低，在高他莫昔芬浓度下流动性增加。可见，文献中关于他莫昔芬对膜流动性影响的明显差异主要源于他莫昔芬的使用浓度以及脂质流动性和脂质顺序概念的混淆。
There are contradictory results in the literature relating to the effect oftamoxifen on membrane fluidity. The present work investigates the effect oftamoxifen on membrane dynamics to find out whether the concentration oftamoxifen can be one of the factors in this discrepancy. Turbidity(absorbance at 440 nm) and Fourier transform infrared spectroscopicstudies reveal that tamoxifen causes opposite effects on membranefluidity at low (1 mol.%) and high (30 mol.%) tamoxifen concentrations. Lowtamoxifen concentrations increase the absorbance in the gel and liquidcrystalline phase, whereas high tamoxifen concentrations decrease theabsorbance in gel and liquid crystalline phase, whereas tamoxifenconcentrations decrease the absorbance. Observations on both phasesshow that the bandwidth of the CH2 stretching bands decreases with1 mol.% tamoxifen and increases with 30 mol.% tamoxifen present, indicatinga decrease in membrane fluidity at low tamoxifen concentrations and anincrease in fluidity at high tamoxifen concentrations. It is seen that theapparent discrepancy in the literature on the effect of tamoxifen onmembrane fluidity mainly arises from the tamoxifen concentration used andthe confusion on the concept of lipid fluidity and lipid order.