In vitro differentiation and mineralization of dental pulp stem cells on enamel-like fluorapatite surfaces.
In vitro differentiation and mineralization of dental pulp stem cells on enamel-like fluorapatite surfaces.
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DOI：
10.1089/ten.tec.2011.0624
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发表时间：
2012-06
期刊：
影响因子：
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通讯作者：
Xiaodong Wang;T. Jin;Syweren Chang;Zhaocheng Zhang;A. Czajka-Jakubowska;J. Nör;B. Clarkson;L. Ni;Jun D Liu
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文献类型：
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作者：
Xiaodong Wang;T. Jin;Syweren Chang;Zhaocheng Zhang;A. Czajka-Jakubowska;J. Nör;B. Clarkson;L. Ni;Jun D Liu
我们以前的研究表明，氟磷灰石（FA）晶体表面的良好的生物相容性，提供了一个有利的环境，为功能的细胞-基质相互作用的人牙髓干细胞（DPSC），也支持他们的长期增长。本研究的目的是进一步研究这种釉质样表面是否可以支持DPSCs的分化和矿化，因此，作为一个潜在的模型，用于研究牙釉质/牙本质界面，也许，牙本质/牙髓再生的牙齿组织工程。人成骨通路聚焦聚合酶链反应（PCR）阵列显示，与蚀刻不锈钢（SSE）表面相比，FA表面上人DPSCs成骨相关基因的表达增加。与PCR阵列一致，FA促进矿化与SSE表面相比，添加或不添加矿化促进补充剂（MS）。碱性磷酸酶（ALP）染色，茜素红染色，四环素染色的矿物质形成证实了这一点。总之，FA晶体表面，特别是有序（OR）FA表面，模仿釉质的物理结构，为细胞提供了有利的细胞外基质微环境。这导致了人DPSCs的分化和矿化组织的形成，因此，表明它可能是一个有前途的仿生模型的牙本质牙髓组织工程。
Our previous studies have shown good biocompatibility of fluorapatite (FA) crystal surfaces in providing a favorable environment for functional cell-matrix interactions of human dental pulp stem cells (DPSCs) and also in supporting their long-term growth. The aim of the current study was to further investigate whether this enamel-like surface can support the differentiation and mineralization of DPSCs, and, therefore, act as a potential model for studying the enamel/dentin interface and, perhaps, dentine/pulp regeneration in tooth tissue engineering. The human pathway-focused osteogenesis polymerase chain reaction (PCR) array demonstrated that the expression of osteogenesis-related genes of human DPSCs was increased on FA surfaces compared with that on etched stainless steel (SSE). Consistent with the PCR array, FA promoted mineralization compared with the SSE surface with or without the addition of a mineralization promoting supplement (MS). This was confirmed by alkaline phosphatase (ALP) staining, Alizarin red staining, and tetracycline staining for mineral formation. In conclusion, FA crystal surfaces, especially ordered (OR) FA surfaces, which mimicked the physical architecture of enamel, provided a favorable extracellular matrix microenvironment for the cells. This resulted in the differentiation of human DPSCs and mineralized tissue formation, and, thus, demonstrated that it may be a promising biomimetic model for dentin-pulp tissue engineering.