CAMP STIMULATES THE C/EBP-RELATED TRANSCRIPTION FACTOR RNFIL-6 TO TRANS-LOCATE TO THE NUCLEUS AND INDUCE C-FOS TRANSCRIPTION
CAMP STIMULATES THE C/EBP-RELATED TRANSCRIPTION FACTOR RNFIL-6 TO TRANS-LOCATE TO THE NUCLEUS AND INDUCE C-FOS TRANSCRIPTION
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DOI：
10.1101/gad.5.10.1754
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发表时间：
1991-10-01
影响因子：
10.5
通讯作者：
ZIFF, E
中科院分区：
文献类型：
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作者：
METZ, R;ZIFF, E
c-fos血清反应元件（SRE）是c-fos启动子的一个多功能调控区，对多种诱导剂有反应。 最近，我们已经证明，SRE结合C/EBP相关的转录因子大鼠NFIL-6（rNFIL-6）。 在这项研究中，我们表明，rNFIL-6的大鼠嗜铬细胞瘤PC 12细胞系中的cAMP第二信使途径的调节。 在毛喉素处理后，rNFIL-6与SRE的结合增加，并且该因子变得磷酸化并经历到细胞核的转位。 在瞬时共转染试验中，rNFIL-6能够以依赖于SRE的方式反式激活c-fos启动子。 这些数据表明，rNFIL-6经历了一种新的激活，其中cAMP诱导的核转位允许rNFIL-6与SRE结合并有助于c-fos激活。 我们认为rNFIL-6是c-fos基因的一个额外的调节成分，它提供cAMP对多功能SRE的反应。
The c-fos serum response element (SRE) is a multifunctional regulatory region of the c-fos promoter that responds to a variety of inducers. Recently, we have demonstrated that the SRE binds the C/EBP-related transcription factor rat NFIL-6 (rNFIL-6). In this study we show that rNFIL-6 is regulated by the cAMP second messenger pathway in the rat pheochromocytoma PC12 cell line. Following forskolin treatment, rNFIL-6 binding to the SRE is increased, and the factor becomes phosphorylated and undergoes a trans-location to the nucleus. In transient cotransfection assays, rNFIL-6 is capable of trans-activating the c-fos promoter in a manner dependent on the SRE. These data show that rNFIL-6 undergoes a novel activation in which cAMP-induced nuclear trans-location allows rNFIL-6 to bind to the SRE and contribute to c-fos activation. We propose that rNFIL-6 is an additional regulatory component of the c-fos gene, which provides cAMP responsiveness to the multifunctional SRE.