A Novel Eukaryote-Like CRISPR Activation Tool in Bacteria: Features and Capabilities
A Novel Eukaryote-Like CRISPR Activation Tool in Bacteria: Features and Capabilities
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DOI：
10.1002/bies.201900252
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发表时间：
2020-04-20
期刊：
影响因子：
4
通讯作者：
Wang, Baojun
中科院分区：
文献类型：
--
作者：
Liu, Yang;Wang, Baojun
细菌中的CRISPR（成簇规则间隔短回文重复序列）激活（CRISPRa）是用于可编程基因激活的有吸引力的方法。最近，已经报道了真核生物样的sigma（54）依赖性CRISPRa系统。它具有高动态范围，并允许灵活的目标位置选择。在此，对CRISPRa在细菌中的现有策略进行了概述，并介绍了CRISPRa系统的特点和设计原理。讨论了应用真核生物类CRISPRa系统的可能场景，并对性能优化和未来功能扩展提出了相应的建议。作者设想了新的真核生物样CRISPRa系统，使多重基因调控的新设计成为可能，并促进了各种生物技术相关或疾病相关细菌物种中sigma（54）依赖性基因调控网络的研究。
CRISPR (clustered regularly interspaced short palindromic repeats) activation (CRISPRa) in bacteria is an attractive method for programmable gene activation. Recently, a eukaryote-like, sigma(54)-dependent CRISPRa system has been reported. It exhibits high dynamic ranges and permits flexible target site selection. Here, an overview of the existing strategies of CRISPRa in bacteria is presented, and the characteristics and design principles of the CRISPRa system are introduced. Possible scenarios for applying the eukaryote-like CRISPRa system is discussed with corresponding suggestions for performance optimization and future functional expansion. The authors envision the new eukaryote-like CRISPRa system enabling novel designs in multiplexed gene regulation and promoting research in the sigma(54)-dependent gene regulatory networks among a variety of biotechnology relevant or disease-associated bacterial species.