Transient gut retention and persistence of Salmonella through metamorphosis in the lesser mealworm, Alphitobius diaperinus (Coleoptera: Tenebrionidae)
Transient gut retention and persistence of Salmonella through metamorphosis in the lesser mealworm, Alphitobius diaperinus (Coleoptera: Tenebrionidae)
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DOI：
10.1111/j.1365-2672.2012.05265.x
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发表时间：
2012-05-01
影响因子：
4
通讯作者：
Yu, Z.
中科院分区：
文献类型：
--
作者：
Crippen, T. L.;Zheng, L.;Yu, Z.
目的：本研究进行了确定保留沙门氏菌通过Alphitobius diaperinus变态和它的贡献，通过排便，外部contamination.Methods和结果：昆虫暴露于一个标记的沙门氏菌enterica和外部消除评估。(i)连续3周，每天用过滤器从受约束的昆虫收集粪便用于分析。(ii)暴露在一个封闭的容器中的幼虫，随后通过化蛹，和新出现的成年人进行了检查，他们保留的标志bacteries.Conclusions：暴露的成人和幼虫产生沙门氏菌阳性粪便平均为8天，从6到11天和6到12天，分别。19%的幼虫携带沙门氏菌通过变态和羽化，与5%的蛹蜕皮是积极的，以及。意义和影响的研究：许多来源的食源性病原体的家禽生产设施，包括水库人口，目前不承认。这削弱了生产者减轻病原体在动物、人类和环境之间转移的能力。家禽管理标准接受废弃物的再利用。在重复利用的垫料上，Alphitobius diaperinus在羊群轮换之间存活，并且在这项研究中证明它们携带的沙门氏菌可以与它们一起存活。
Aims: This study was undertaken to determine the retention of Salmonella through Alphitobius diaperinus metamorphosis and its contribution, through defecation, to external contamination.Methods and Results: Insects were exposed to a tagged Salmonella enterica and evaluated for external elimination. (i) Each day for 3 weeks, a filter collected frass from a restrained insect for analysis. (ii) Exposed larvae in a closed container were followed through pupation, and newly emerged adults were examined for their retention of marker bacteria.Conclusions: Exposed adults and larvae produced Salmonella-positive frass for an average of 8 days, ranging from 6 to 11 days and 6 to 12 days, respectively. Nineteen per cent of the larvae carried Salmonella through metamorphosis and eclosion, with 5% of the pupal exuviae being positive as well.Significance and Impact of the Study: Many sources of foodborne pathogens within the poultry production facilities, including reservoir populations, currently go unrecognized. This diminishes the ability of producers to mitigate the transfer of pathogens between animals, humans and the environment. Poultry management standards accept the reutilization of litter. Alphitobius diaperinus survive between flock rotations on the reutilized litter, and it was demonstrated in this study that the Salmonella they carry can survive with them.