Histological features of oral epithelium in seven animal species: As a reference for selecting animal models
Histological features of oral epithelium in seven animal species: As a reference for selecting animal models
复制标题
七种动物口腔上皮的组织学特征：为选择动物模型提供参考
DOI：
10.1016/j.ejps.2015.09.019
复制
发表时间：
2016-01-01
影响因子：
4.6
通讯作者：
Zhao, Yifang
中科院分区：
文献类型：
--
作者：
Sa, Guoliang;Xiong, Xuepeng;Zhao, Yifang
几种动物已被用作口腔粘膜基础和临床研究的模型。很少有研究集中在选择合适的动物模型。本研究旨在为选择潜在的模型提供组织学参考。通过探索6种形态学特征和2种免疫组化标记物来评估组织学特征。形态学特征包括角化、基底膜外观、上皮厚度、网脊长度、相邻网脊距离和区域变化;免疫组化标记物包括Ki 67（增殖标记物）和细胞角蛋白19（CK 19;干性标记物）。根据指定的评分标准计算每个种属与人类相比的组织学相似性。结果表明，狗和猪的颊粘膜均未角化，与人的颊粘膜相比，颊粘膜的网嵴长度和距离相似。犬、大鼠和豚鼠粘膜在基底膜中具有相似的大体外观。犬口腔粘膜上皮厚度与人口腔粘膜上皮厚度相似。与人粘膜相比，犬、猪、大鼠和兔粘膜表现出相应的区域变化。Ki 67阳性细胞在人和犬的粘膜主要定位于基底上层，而大多数增殖细胞在基底层在其他物种。CK 19免疫反应仅在人和犬粘膜中检测到。犬粘膜获得最高点值（14），而猪、大鼠、兔、豚鼠、绵羊和布法罗粘膜的评分分别为8、6、5、5、5和2。不同动物种属的口腔上皮组织学差异相当大;犬的粘膜与人的粘膜最为相似，暗示其作为动物模型的组织学基础。(C)2015 Elsevier B. V.版权所有。
Several animals have been used as models for basic and clinical research on oral mucosa. Few studies have focused on the selection of an appropriate animal model. This study aimed to provide histological references for selecting a potential model. Histological features were assessed by exploring 6 morphological characteristics and 2 immunohistochemical markers. The morphological characteristics included keratinization, basal membrane appearance, epithelial thickness, rete ridge length, adjacent rete ridge distance, and regional variation; the immunohistochemical markers included Ki67 (a proliferative marker) and Cytokeratin 19 (CK19; a stemness marker). The histological similarity of each species compared to humans was calculated according to the designated scoring criteria. The results showed that the buccal mucosae from dog and pig were non-keratinized, with similar rete ridge length and distance, compared to that of humans. The dog, rat, and cavy mucosae had analogous gross appearances in the basal membrane. The dog oral mucosae shared similar epithelial thickness with human oral mucosae. Compared to the human mucosa, the dog, pig, rat, and rabbit mucosae exhibited corresponding regional variations. The Ki67-positive cells in human and canine mucosae were predominantly localized in the suprabasal layers, whereas most of the proliferative cells were in the basal layer in other species. CK19 immunoreactivities were detected only in human and canine mucosae. The canine mucosae gained the highest point value ( 14), whereas the scores for the pig, rat, rabbit, cavy, sheep, and buffalo mucosae were 8, 6, 5, 5, 5, and 2, respectively. The histological variations in the oral epithelium of diverse animal species are considerable; the mucosae from dogs are most similar to human mucosae, implicating its histological basis as an animal model. (C) 2015 Elsevier B.V. All rights reserved.