Recovery of Staphylococcus aureus from centrifuged quarter milk samples.
Recovery of Staphylococcus aureus from centrifuged quarter milk samples.
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从离心的四分之一牛奶样品中回收金黄色葡萄球菌。
DOI：
10.3168/jds.s0022-0302(97)76273-8
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发表时间：
1997
影响因子：
3.5
通讯作者：
G. Ruffo
中科院分区：
文献类型：
--
作者：
A. Zecconi;R. Piccinini;A. Zepponi;G. Ruffo
在野外条件下，很难鉴定出对金黄色葡萄球菌引起的乳腺炎呈阳性的奶牛。将从四分之一牛奶样品中分离金黄色葡萄球菌的频率与离心这些相同样品后从沉积物中回收金黄色葡萄球菌的频率进行比较。总体而言，研究人员对来自 194 头奶牛的 776 个四分之一牛奶样本进行了研究。从 82 个样本中获得金黄色葡萄球菌阳性培养物；来自四分之一牛奶样品的 153 个沉积物也呈金黄色葡萄球菌阳性。这项调查的结果表明，四分之一牛奶样本沉淀物的培养使阳性结果的数量增加了 145.5%，具体取决于牛群。使用不同组的样品，包括产犊后 1 至 5 天或 7 至 10 天采集的样品以及乳房内治疗后采集的样品，发现与未离心的同一季度牛奶样品的培养物相比，离心后金黄色葡萄球菌阳性的培养物增加了 94%。沉淀培养物可用于金黄色葡萄球菌控制计划，该计划需要隔离、选择性处理或扑杀金黄色葡萄球菌呈阳性的奶牛。
The identification of cows that are positive for mastitis caused by Staphylococcus aureus is difficult under field conditions. The frequency of isolation of S. aureus from quarter milk samples was compared with the frequency of recovery of S. aureus from sediment after centrifugation of those same samples. Overall, 776 quarter milk samples from 194 cows were studied. Cultures that were positive for S. aureus were obtained from 82 samples; 153 sediments from quarter milk samples were also positive for S. aureus. The results of this investigation showed that cultures of the sediment of quarter milk samples increased the number of positive outcomes up to 145.5%, depending on the herd. Using a different group of samples, including samples taken 1 to 5 d or 7 to 10 d after calving and samples taken after intramammary therapy, a 94% increase in cultures that were positive for S. aureus after centrifugation was found compared with cultures of the same quarter milk samples that were not centrifuged. Sedimented cultures may be useful in S. aureus control programs that require the segregation, selective treatment, or culling of cows that are positive for S. aureus.