Transient postoperative vascular endothelial growth factor (VEGF)-neutralisation improves graft survival in corneas with partly regressed inflammatory neovascularisation
Transient postoperative vascular endothelial growth factor (VEGF)-neutralisation improves graft survival in corneas with partly regressed inflammatory neovascularisation
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DOI：
10.1136/bjo.2008.145128
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发表时间：
2009-08-01
影响因子：
4.1
通讯作者：
Cursiefen, C.
中科院分区：
文献类型：
--
作者：
Bachmann, B. O.;Luetjen-Drecoll, E.;Cursiefen, C.
背景资料：高危角膜移植术通常在无炎症和静止期后进行，角膜的血管和淋巴管部分消退。我们分析了在具有部分退化角膜血管（“中等风险”）的小鼠中抑制角膜移植术后血管再生是否改善移植物存活率。方法：在Balb/c小鼠（6-8周龄）的角膜中放置三个间断的基质缝线（11-0）6周。拆线后6个月，用C57 BL/6供体进行穿透性角膜移植术。治疗组在角膜移植术后第0、4、7和14天腹膜内接受血管内皮生长因子-A特异性细胞因子捕获物（VEGF捕获物）（每只小鼠25 mg/kg;对照接受等量的Fc蛋白）。病理血管生成和淋巴管生成之前，以及3天或8周后角膜移植术和移植物survival.Results：角膜移植术后3天角膜血运重建充分减少VEGF陷阱（血管化面积减少42.7%，淋巴血管化面积减少54.7%）。角膜移植术后8周的存活率，治疗组为36%，对照组为9%（n = 11; p
Background: High-risk keratoplasties are usually performed after an uninflamed and quiescent interval in corneas with partly regressed blood and lymphatic vessels. We analysed whether the inhibition of post-keratoplasty revascularisation in mice with partly regressed corneal vessels ("intermediate-risk'') improves graft survival.Methods: Three interrupted stromal sutures (11-0) in corneas of Balb/c mice (6-8 weeks old) were placed for 6 weeks. Six months after suture removal, penetrating keratoplasty was performed with C57BL/6 donors. The treatment group received a vascular endothelial growth factor-A specific cytokine trap (VEGF Trap) intraperitoneally at days 0, 4, 7 and 14 after keratoplasty (25 mg/kg per mouse; controls received equal amounts of Fc protein). Pathological haemangiogenesis and lymphangiogenesis prior to as well as 3 days or 8 weeks after keratoplasty and graft survival were analysed.Results: Three days after keratoplasty corneal revascularisation was sufficiently reduced by VEGF Trap (haemvascularised areas 42.7% reduction; lymph-vascularised areas 54.7% reduction). Survival proportions 8 weeks after keratoplasty were 36% in the treatment group compared with 9% in the control group (n = 11; p