Modulome-Fangjiome Association Study (MoFAS) reveals differential target distribution among four similar fangjis (formulas).
Modulome-Fangjiome Association Study (MoFAS) reveals differential target distribution among four similar fangjis (formulas).
复制标题
Modulome-Fangjiome关联研究（MoFAS）揭示了四个相似的fangjis（公式）之间的差异目标分布。
DOI：
10.1016/j.jep.2021.113822
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发表时间：
2021-01
影响因子：
5.4
通讯作者：
Wang Zhong
中科院分区：
文献类型：
--
作者：
Wang Pengqian;Zhou Weiwei;Liu Jun;Sui Feng;Wang Zhong
准确的靶向分布是防棘药物合理应用的关键（配方）具有相似的功效和草药成分，以最大限度地提高功效，最小限度地降低毒性。研究的目的为了开发一种算法，用于调查不同的靶标分布和特征机制。主要方法在这项研究中，本研究提出了一个模块-方剂组关联研究（Modulome-FangjiomeAssociation Study，MoFAS），用于比较益气养血方剂组（以四种方剂：FEJ、SDT、LYG和QOL为代表）的方剂。首先，将这4个方集的数据库驱动目标网络构造为气血网络，并将其分解为模块。在此基础上，基于一致性评分和丰富度分析，构建了功能景观模块图.最后，采用靶向率矩阵（TR）评价该方剂对模块组（一组模块）的贡献，并采用主成分分析（PCA）比较方剂的特征效应。在功能景观中，3个主要模块主要涉及内分泌系统和环境适应。在靶点分布上，SDT与QOL较为接近，FEJ与LYG在主成分分析中与其他方剂的位置较远。FEJ、SDT和QOL的共同作用集中在环境扰动中的应激反应和生物体发育，但FEJ在调节关键靶点方面更上级，主要集中在激素和神经递质过程。SDT和QOL主要集中在气血网络的大部分尺度上，尤其是细胞有丝分裂周期和发育。结论本研究首次探讨了益气养血方药的靶点分布差异，为益气养血方药的特色疗效提供了直接证据，为方剂的精准应用提供了依据，为方剂的合理用药人群提供了依据。
Ethnopharmacological relevancePrecise target distribution is a key issue for further appropriate applications of fangjis (formulas) with similar efficacy and herbal constituents to maximize efficacy and minimize toxicity.Aim of the studyTo develop an algorithm for investigating the differential target distributions and characteristic mechanisms of fangjis.Main methodsIn this study, we proposed a Modulome-Fangjiome Association Study (MoFAS) for comparing fangjis from qi-invigorating and xue-nourishing fangjiome (represented by four fangjis: FEJ, SDT, LYG and QOL). Firstly, the database-driven target network of these 4 fangjis was constructed as qi-xue network and decomposed into modules. Then, the modular map with functional landscape were constructed based on consistency score and enrichment analysis. Finally, we employed a targeting rate (TR) matrix to assess the contribution of this fangjiome to modulome (a set of modules) and compared characteristic effect of fangjis by principal component analysis (PCA).ResultsA qi-xue network constituted by 579 proteins and 23 modules were constructed. In the functional landscape, 3 primary modules were mainly involved in the endocrine system and environmental adaptation. For the target distribution, SDT and QOL were more similar; the FEJ and LYG were located distant from other fangjis according to PCA. The common effects of FEJ, SDT, and QOL focused on stress response and organism development in environmental perturbation, but the FEJ was superior in regulating critical targets, primarily focusing on hormone and neurotransmitter processes. SDT and QOL were concentrated on the majority scale of the qi-xue network, especially for the mitotic cell cycle and development. LYG only targeted lymphocyte costimulation and icosanoid biosynthetic processes.ConclusionIn this study, for the first time, we investigated the difference in the target distribution of qi-invigorating and xue-nourishing fangjiome and provided direct evidence for the characteristic therapeutic effect of these fangjis, which may promote the precise application of fangjis and support the identification of appropriate populations.