Spatial and temporal structuring of arbuscular mycorrhizal communities is differentially influenced by abiotic factors and host crop in a semi-arid prairie agroecosystem
Spatial and temporal structuring of arbuscular mycorrhizal communities is differentially influenced by abiotic factors and host crop in a semi-arid prairie agroecosystem
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DOI：
10.1111/1574-6941.12300
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发表时间：
2014-05-01
影响因子：
4.2
通讯作者：
Gan, Yantai
中科院分区：
文献类型：
--
作者：
Bainard, Luke D.;Bainard, Jillian D.;Gan, Yantai
农业生态系统是一个动态的系统，经常经历化学投入和植物覆盖的变化。本研究的目的是测试是否非生物（土壤化学性质和气候）和生物（植物宿主身份）因素影响的空间和时间结构的丛枝菌根真菌（AMF）在半干旱草原农业生态系统的社区。454 GS FLX+高通量测序技术成功地用于基于长读段（平均长度：751.7bp）的AMF群落的表征，并产生具有优异分类覆盖的高分辨率数据。AMF社区的组成殖民根的三种作物（豌豆，扁豆，小麦）显着不同，但植物宿主身份有一个最小的影响AMF社区的组成在土壤中。我们观察到AMF社区的组成在作物的根和周围的土壤在生长季节的时间转变。这种时间上的变化在与根相关的AMF群落中尤为明显，并与土壤磷通量和气候变量相关。与此相反，AMF社区在网站的空间结构与土壤pH值和电导率。个别AMF类群与pH值，电导率和磷酸盐通量显着相关，这些关系在属的水平上在球囊菌门内是遗传保守的。
Agroecosystems are dynamic systems that experience frequent chemical inputs and changes in plant cover. The objective of this study was to test whether abiotic (soil chemical properties and climate) and biotic (plant host identity) factors influence the spatial and temporal structuring of arbuscular mycorrhizal fungal (AMF) communities in a semi-arid prairie agroecosystem. 454 GS FLX+ high-throughput sequencing technology was successfully utilized to characterize the AMF communities based on long reads (mean length: 751.7bp) and generated high-resolution data with excellent taxonomic coverage. The composition of the AMF community colonizing roots of the three crops (pea, lentil, and wheat) significantly differed, but plant host identity had a minimal effect on the composition of the AMF community in the soil. We observed a temporal shift in the composition of AMF communities in the roots and surrounding soil of the crops during the growing season. This temporal shift was particularly evident in the root-associated AMF community and was correlated with soil phosphate flux and climatic variables. In contrast, the spatial structuring of the AMF community in the site was correlated with soil pH and electrical conductivity. Individual AMF taxa were significantly correlated with pH, electrical conductivity, and phosphate flux, and these relationships were phylogenetically conserved at the genus level within the Glomeromycota.