THE SACCHAROMYCES-CEREVISIAE NUCLEAR GENE SUV3 ENCODING A PUTATIVE RNA HELICASE IS NECESSARY FOR THE STABILITY OF MITOCHONDRIAL TRANSCRIPTS CONTAINING MULTIPLE INTRONS
THE SACCHAROMYCES-CEREVISIAE NUCLEAR GENE SUV3 ENCODING A PUTATIVE RNA HELICASE IS NECESSARY FOR THE STABILITY OF MITOCHONDRIAL TRANSCRIPTS CONTAINING MULTIPLE INTRONS
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DOI：
10.1007/bf00309780
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发表时间：
1995-08-01
期刊：
影响因子：
2.5
通讯作者：
LAZOWSKA, J
中科院分区：
文献类型：
--
作者：
GOLIK, P;SZCZEPANEK, T;LAZOWSKA, J
核基因SUV 3的产物与酵母线粒体中的多种转录后过程有关。我们分析了SUV 3基因破坏对线粒体cytb和cox 1基因含内含子等位基因表达的影响。我们已经构建了几个菌株的线粒体基因组含有不同的组合cytb和cox1内含子，并与这些基因组的破坏SUV 3。测试所得菌株的呼吸能力和光谱细胞色素含量。所有只含有两个或三个内含子的菌株都显示cytb和cox 1的正常表达，而含有更多内含子的菌株则不能表达相应的基因。通过北方杂交分析线粒体RNA，发现含内含子较多的菌株呼吸活性的丧失是由于mRNA水平的降低，而没有高分子量前体的过度积累。然而，基因的转录没有受到影响。这些结果使我们的概念，SUV 3是所需的含内含子的cytb和cox 1转录的稳定性在一个累积的方式，不依赖于任何特定的内含子。
The product of the nuclear gene SUV3 is implicated in a variety of post-transcriptional processes in yeast mitochondria. We have analysed the effect of SUV3 gene-disruption on the expression of intron-containing alleles of the mitochondrial cytb and cox1 genes. We have constructed several strains with mitochondrial genomes containing different combinations of cytb and cox1 introns, and associated these genomes with the disruption of SUV3. The resulting strains were tested for their respiratory competence and spectral cytochrome content. All the strains containing only two or three introns showed normal expression of cytb and cox1, whereas the strains containing more introns were unable to express the appropriate gene. The analysis of mitochondrial RNAs by Northern hybridisation showed that the loss of respiratory competence in the strains containing more introns is due to the decrease of mRNA level with no over-accumulation of high-molecular-weight precursors. However, the transcription of the genes was not affected. These results led us to the notion that SUV3 is required for the stability of intron-containing cytb and cox1 transcripts in a cumulative way, not dependent on any particular intron.